Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 2223686..2224332 Replicon chromosome
Accession NZ_AP024515
Organism Nitrosospira sp. NRS527

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag KJS40_RS10000 Protein ID WP_280520233.1
Coordinates 2224015..2224332 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag KJS40_RS09995 Protein ID WP_213537442.1
Coordinates 2223686..2223988 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KJS40_RS09970 (NNRS527_02113) 2219298..2219453 + 156 WP_213537434.1 hypothetical protein -
KJS40_RS09975 (NNRS527_02114) 2219508..2220161 - 654 WP_213537436.1 PEP-CTERM sorting domain-containing protein -
KJS40_RS09985 (NNRS527_02116) 2221239..2221595 + 357 WP_213537438.1 DsrE family protein -
KJS40_RS09990 (NNRS527_02117) 2221635..2222645 - 1011 WP_213537440.1 zinc-dependent alcohol dehydrogenase family protein -
KJS40_RS09995 (NNRS527_02118) 2223686..2223988 - 303 WP_213537442.1 HigA family addiction module antitoxin Antitoxin
KJS40_RS10000 (NNRS527_02119) 2224015..2224332 - 318 WP_280520233.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KJS40_RS10010 (NNRS527_02123) 2225700..2226820 + 1121 WP_213537446.1 IS3 family transposase -
KJS40_RS10020 (NNRS527_02125) 2227506..2228735 - 1230 WP_213537448.1 aspartate kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(17-105)

Antitoxin

Cro/C1-type helix-turn-helix domain

(9-64)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12174.88 Da        Isoelectric Point: 9.7799

>T38548 WP_280520233.1 NZ_AP024515:c2224332-2224015 [Nitrosospira sp. NRS527]
MTIDVQRQSVLGLRMIKSFKCRKTERLFEGQHIAAFSGFKRQAEKRLRILDAADTLEALAALPSNRFERLSGDRAGQYSI
RVNQQWRVCFTWNEGANDVEIVDYH

Download         Length: 318 bp

>T38548 NZ_AP024515:c2224332-2224015 [Nitrosospira sp. NRS527]
TTGACGATTGACGTTCAGCGTCAATCTGTGCTAGGATTAAGAATGATAAAGAGCTTTAAATGCCGAAAAACAGAGCGCCT
CTTTGAAGGCCAGCATATTGCGGCGTTTTCCGGCTTTAAACGCCAAGCGGAGAAGCGCCTTAGAATACTGGATGCTGCCG
ATACACTTGAAGCGTTGGCTGCTTTACCCAGTAACCGCTTTGAGCGCTTATCCGGTGATAGAGCGGGACAATACAGCATC
CGCGTCAATCAACAATGGCGTGTATGTTTTACGTGGAACGAAGGTGCGAATGATGTGGAAATCGTGGATTATCACTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11553.31 Da        Isoelectric Point: 6.8824

>AT38548 WP_213537442.1 NZ_AP024515:c2223988-2223686 [Nitrosospira sp. NRS527]
MTREAIHPGEFLADELEEIGMTRTELARQIDVPPNRISQLIHGKRDLTADTALRLGRFFGTGPELWLNLQKTYELDKAKI
ELGKKIQKINPWKPHEEVHI

Download         Length: 303 bp

>AT38548 NZ_AP024515:c2223988-2223686 [Nitrosospira sp. NRS527]
ATGACGAGAGAAGCTATTCACCCTGGCGAATTTCTTGCTGATGAATTGGAAGAAATCGGTATGACGCGAACAGAGCTTGC
GCGGCAAATCGATGTGCCTCCAAACCGTATCTCGCAGCTTATTCATGGAAAACGCGACTTGACAGCAGATACAGCGCTAC
GGTTAGGACGGTTCTTTGGCACCGGCCCGGAACTGTGGTTGAACCTGCAAAAAACCTATGAGCTTGATAAAGCGAAAATT
GAGCTGGGTAAAAAAATCCAGAAAATCAATCCGTGGAAACCCCACGAAGAAGTACATATTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References