Detailed information of TA system TA038546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 5361084..5361679
• Replicon: chromosome
• Accession: NZ_AP024513
• Organism: Pseudomonas aeruginosa isolate Pa12

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: KJS76_RS24705
• Protein ID: WP_003113526.1
• Coordinates: 5361401..5361679 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KJS76_RS24700
• Protein ID: WP_003099268.1
• Coordinates: 5361084..5361389 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS76_RS24665 (RVB2_49060)	5356234..5357082	+	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-
KJS76_RS24675 (RVB2_49070)	5357249..5358190	+	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
KJS76_RS24680 (RVB2_49080)	5358307..5358921	+	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
KJS76_RS24685 (RVB2_49090)	5358963..5359547	+	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
KJS76_RS24690 (RVB2_49100)	5359588..5360688	+	1101	WP_003099270.1	redox-regulated ATPase YchF	-
KJS76_RS24700 (RVB2_49110)	5361084..5361389	-	306	WP_003099268.1	HigA family addiction module antitoxin	Antitoxin
KJS76_RS24705	5361401..5361679	-	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KJS76_RS29535	5361732..5361860	-	129	Protein_4891	integrase	-
KJS76_RS24710 (RVB2_49120)	5362008..5364236	+	2229	WP_023090880.1	TonB-dependent receptor	-
KJS76_RS24715 (RVB2_49130)	5364306..5364953	-	648	WP_003095021.1	carbonate dehydratase	-
KJS76_RS24720 (RVB2_49140)	5365015..5366253	-	1239	WP_003113524.1	dipeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T38546 WP_003113526.1 NZ_AP024513:c5361679-5361401 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T38546 NZ_AP024513:c5361679-5361401 [Pseudomonas aeruginosa]
ATGATTCTGACCTTTCGCTGCGACGAGACTCGTCAGCTTTTTGAGACGGGTCTTTCGAGGCGGTGGGGAGCGATCCTCAC
AGTCGCTACGCGTAAGCTCGCAATGCTTCATGCGGCTACGGAGCTTCGAGACCTGCGCTCTCCACCTGGAAACCGGTTGG
AGCCGTTGCAGGGAAAGCGGGCGGGCCAACATAGCATCAGGATCAATGACCAGTGGCGTGTCTGTTTCGTCTGGACGGAT
GCGGGTCCCGAAGAAGTCGAAATAGTTGATTACCACTGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT38546 WP_003099268.1 NZ_AP024513:c5361389-5361084 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT38546 NZ_AP024513:c5361389-5361084 [Pseudomonas aeruginosa]
ATGGCTACCAATGGTATGCGCCCCATCCATCCTGGGGAAATATTGCGCGATGAGTTTCTGATGGAGTTGGATATCTCTCC
AGCTGCTCTAGCACGCGCTTTGAAAGTCTCCGCTCCGACAGTGAACGATATCGTTCGTGAGCAGCGTGGTATCTCCGCAG
ATATGGCGATTCGTCTGGGGCGTTACTTTGATACGTCCGCTCAGTTCTGGATGAATCTCCAGAGTGAGTATTCGTTAGCA
ACTGCTTATGCGGCGAATGGCAAGCAGATCGAACATGAGATTGAGCCGCTGCTTGCTCACGGATAG

Structures

Toxin

• AlphaFold DB: V6ALY3