Detailed information of TA system TA038527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 556948..557362
• Replicon: chromosome
• Accession: NZ_AP024511
• Organism: Staphylococcus aureus isolate 2007-13

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KJS82_RS03135
• Protein ID: WP_031267622.1
• Coordinates: 556948..557199 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KJS82_RS03140
• Protein ID: WP_000028424.1
• Coordinates: 557189..557362 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS82_RS03085 (552025)	552025..552582	+	558	WP_000645042.1	DUF2815 family protein	-
KJS82_RS03090 (552651)	552651..554603	+	1953	WP_213607992.1	DNA polymerase	-
KJS82_RS03095 (554616)	554616..554801	+	186	WP_001164629.1	DUF3113 family protein	-
KJS82_RS03100 (554801)	554801..555202	+	402	WP_000113974.1	PVL family protein	-
KJS82_RS03105 (555202)	555202..555456	+	255	WP_000022736.1	DUF3310 domain-containing protein	-
KJS82_RS03110 (555456)	555456..555704	+	249	WP_060474161.1	SAV1978 family virulence-associated passenger protein	-
KJS82_RS03115 (555718)	555718..555924	+	207	WP_000693989.1	hypothetical protein	-
KJS82_RS03120 (555927)	555927..556307	+	381	WP_001668982.1	hypothetical protein	-
KJS82_RS03125 (556304)	556304..556651	+	348	WP_000979209.1	YopX family protein	-
KJS82_RS03130 (556648)	556648..556955	+	308	Protein_588	hypothetical protein	-
KJS82_RS03135 (556948)	556948..557199	+	252	WP_031267622.1	DUF1024 family protein	Toxin
KJS82_RS03140 (557189)	557189..557362	+	174	WP_000028424.1	hypothetical protein	Antitoxin
KJS82_RS03145 (557363)	557363..557524	+	162	WP_000889684.1	hypothetical protein	-
KJS82_RS03150 (557539)	557539..558072	+	534	WP_063653166.1	dUTPase	-
KJS82_RS03155 (558109)	558109..558315	+	207	WP_000195810.1	DUF1381 domain-containing protein	-
KJS82_RS03160 (558312)	558312..558464	+	153	WP_000595263.1	transcriptional activator RinB	-
KJS82_RS03165 (558532)	558532..558732	+	201	WP_000265252.1	DUF1514 family protein	-
KJS82_RS03170 (559020)	559020..561467	+	2448	WP_213607959.1	virulence-associated E family protein	-
KJS82_RS03175 (561576)	561576..561674	-	99	WP_001801650.1	hypothetical protein	-
KJS82_RS03180 (561808)	561808..562098	+	291	WP_000665203.1	VRR-NUC domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9419.35 Da
• Isoelectric Point: 3.9476

>T38527 WP_031267622.1 NZ_AP024511:556948-557199 [Staphylococcus aureus]
MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T38527 NZ_AP024511:556948-557199 [Staphylococcus aureus]
ATGAATAATCGCGAAAAAATCGAACAGTCCGTTATTAGTGCTAGTGCGTATAACGGTAATGACACAGAGGGATTGCTAAA
AGAGATTGAGGACGTGTATAAGAAAGCACAAGCGTTTGATGAAATACTTGAGGGAATGACAAATGCTATTCAACATTCAG
TTAAAGAAGGTATTGAACTTGATGAAGCAGTAGGGATTATGACGGGGCAAGTGGTCTATAAATATGAGGAGGAGCAGGAA
AATGAGTATTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT38527 WP_000028424.1 NZ_AP024511:557189-557362 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT38527 NZ_AP024511:557189-557362 [Staphylococcus aureus]
ATGAGTATTAGTGTAGGAGATAAAGTATATAACCATGAAACAAACGAAAGTCTAGAGATTGTGCAATTGGTCGGAGATAT
TAGAGATACACATTATAAACTGTCTGATGATTCAGTTATTAGCATTATAGATTTTATTACTAAACCAATTTATCTAATTA
AGGGGGACGAGTAA