Detailed information of TA system TA038497

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40846..41272
• Replicon: plasmid pEN30S
• Accession: NZ_AP024499
• Organism: Enterobacter asburiae strain En30

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: R2TS_RS22940
• Protein ID: WP_001312861.1
• Coordinates: 40846..41004 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	41048..41272 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R2TS_RS22905	35871..36098	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
R2TS_RS22910	36248..36904	-	657	WP_000283562.1	conjugal transfer transcriptional regulator TraJ	-
R2TS_RS22915	37098..37481	-	384	WP_001354030.1	relaxosome protein TraM	-
R2TS_RS22920	37804..38406	+	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
R2TS_RS22925	38703..39524	-	822	WP_001234445.1	DUF945 domain-containing protein	-
R2TS_RS22930	39635..39931	-	297	WP_001272251.1	hypothetical protein	-
R2TS_RS22935	40231..40608	+	378	Protein_48	hypothetical protein	-
R2TS_RS22940	40846..41004	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	41048..41272	-	225	NuclAT_0	-	Antitoxin
-	41048..41272	-	225	NuclAT_0	-	Antitoxin
-	41048..41272	-	225	NuclAT_0	-	Antitoxin
-	41048..41272	-	225	NuclAT_0	-	Antitoxin
R2TS_RS22945	41284..42003	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
R2TS_RS22950	42000..42434	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
R2TS_RS22955	42503..44471	-	1969	Protein_52	ParB/RepB/Spo0J family partition protein	-
R2TS_RS22960	44532..44765	-	234	WP_000006003.1	DUF905 family protein	-
R2TS_RS22965	44823..45350	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIa / mph(A) / sul1 / blaDHA-1 / qnrB4	-	1..85172	85172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T38497 WP_001312861.1 NZ_AP024499:c41004-40846 [Enterobacter asburiae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T38497 NZ_AP024499:c41004-40846 [Enterobacter asburiae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT38497 NZ_AP024499:c41272-41048 [Enterobacter asburiae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin