Detailed information of TA system TA038494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3660933..3661590
• Replicon: chromosome
• Accession: NZ_AP024498
• Organism: Enterobacter asburiae strain En30

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A808IF03
• Locus tag: R2TS_RS17570
• Protein ID: WP_033146338.1
• Coordinates: 3660933..3661232 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: R2TS_RS17575
• Protein ID: WP_182072757.1
• Coordinates: 3661243..3661590 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R2TS_RS17560	3657303..3659501	+	2199	WP_182072755.1	type I secretion system permease/ATPase	-
R2TS_RS17565	3659512..3660681	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
R2TS_RS17570	3660933..3661232	+	300	WP_033146338.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
R2TS_RS17575	3661243..3661590	+	348	WP_182072757.1	HigA family addiction module antidote protein	Antitoxin
R2TS_RS17580	3661635..3662060	+	426	WP_182072759.1	nucleoside triphosphatase NudI	-
R2TS_RS17585	3662032..3662763	-	732	WP_207167826.1	helix-turn-helix domain-containing protein	-
R2TS_RS17590	3662861..3663280	+	420	WP_182072763.1	nuclear transport factor 2 family protein	-
R2TS_RS17595	3663283..3663801	-	519	WP_182072765.1	hypothetical protein	-
R2TS_RS17600	3663886..3665067	-	1182	WP_048979785.1	PLP-dependent aminotransferase family protein	-
R2TS_RS17605	3665088..3665975	-	888	WP_182072767.1	LysR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11691.32 Da
• Isoelectric Point: 10.0346

>T38494 WP_033146338.1 NZ_AP024498:3660933-3661232 [Enterobacter asburiae]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T38494 NZ_AP024498:3660933-3661232 [Enterobacter asburiae]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13051.01 Da
• Isoelectric Point: 5.8506

>AT38494 WP_182072757.1 NZ_AP024498:3661243-3661590 [Enterobacter asburiae]
MTLQQALRIPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT38494 NZ_AP024498:3661243-3661590 [Enterobacter asburiae]
ATGACACTTCAACAGGCACTCCGCATACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTGA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Structures

Toxin

• AlphaFold DB: A0A808IF03