Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2936191..2936851 Replicon chromosome
Accession NZ_AP024498
Organism Enterobacter asburiae strain En30

Toxin (Protein)


Gene name higB Uniprot ID A0A808IFD9
Locus tag R2TS_RS14135 Protein ID WP_048980127.1
Coordinates 2936498..2936851 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1Z3MX79
Locus tag R2TS_RS14130 Protein ID WP_029741460.1
Coordinates 2936191..2936493 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R2TS_RS14100 2931298..2933487 + 2190 WP_047061141.1 TonB-dependent siderophore receptor -
R2TS_RS14110 2933782..2934114 - 333 WP_058841450.1 type II toxin-antitoxin system PemK/MazF family toxin -
R2TS_RS14115 2934115..2934372 - 258 WP_058841451.1 hypothetical protein -
R2TS_RS14120 2934664..2935053 - 390 WP_096150847.1 RidA family protein -
R2TS_RS14125 2935195..2936115 + 921 WP_048980124.1 LysR family transcriptional regulator -
R2TS_RS14130 2936191..2936493 - 303 WP_029741460.1 XRE family transcriptional regulator Antitoxin
R2TS_RS14135 2936498..2936851 - 354 WP_048980127.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
R2TS_RS14140 2937029..2937922 - 894 WP_048980130.1 LysR family transcriptional regulator -
R2TS_RS14145 2938093..2938701 + 609 WP_080973612.1 glutathione S-transferase family protein -
R2TS_RS14150 2938745..2939695 - 951 WP_023336219.1 HTH-type transcriptional regulator Cbl -
R2TS_RS14155 2939792..2940709 - 918 WP_033145933.1 nitrogen assimilation transcriptional regulator NAC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2934115..2948560 14445


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13599.44 Da        Isoelectric Point: 8.9620

>T38493 WP_048980127.1 NZ_AP024498:c2936851-2936498 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRYSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREFTHWLDRLKERE

Download         Length: 354 bp

>T38493 NZ_AP024498:c2936851-2936498 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTTACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGCACGGGTATC
GTGCTTTGTGCGGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCGGGAATTCAC
TCACTGGCTGGATAGATTAAAAGAGAGGGAATAG

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11050.83 Da        Isoelectric Point: 5.7316

>AT38493 WP_029741460.1 NZ_AP024498:c2936493-2936191 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT38493 NZ_AP024498:c2936493-2936191 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGCGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCTGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGATGGCTCGCACTATGAGTTTGTGCTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IFD9

Antitoxin

Source ID Structure
AlphaFold DB A0A1Z3MX79

References