Detailed information of TA system TA038444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 1291922..1292591
• Replicon: chromosome
• Accession: NZ_AP024414
• Organism: Haemophilus influenzae strain TA8730

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A2R3G0F4
• Locus tag: JUD77_RS06460
• Protein ID: WP_015701504.1
• Coordinates: 1291922..1292305 (+)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A502K5Z3
• Locus tag: JUD77_RS06465
• Protein ID: WP_005651224.1
• Coordinates: 1292289..1292591 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JUD77_RS06425	1288676..1289005	+	330	WP_203620459.1	DUF2190 family protein	-
JUD77_RS06430	1288986..1289297	+	312	WP_015701509.1	hypothetical protein	-
JUD77_RS06435	1289300..1289830	+	531	WP_015701508.1	phage tail protein	-
JUD77_RS06440	1289839..1290249	+	411	WP_005651206.1	phage tail protein	-
JUD77_RS06445	1290252..1290902	+	651	WP_114877444.1	hypothetical protein	-
JUD77_RS06450	1291177..1291584	+	408	WP_114877446.1	hypothetical protein	-
JUD77_RS06455	1291629..1291853	+	225	WP_005651221.1	DUF4035 domain-containing protein	-
JUD77_RS06460	1291922..1292305	+	384	WP_015701504.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JUD77_RS06465	1292289..1292591	+	303	WP_005651224.1	XRE family transcriptional regulator	Antitoxin
JUD77_RS06470	1292607..1292843	+	237	WP_101494098.1	hypothetical protein	-
JUD77_RS06475	1292895..1294823	+	1929	WP_114877448.1	tape measure protein	-
JUD77_RS06480	1294793..1295167	+	375	WP_114877450.1	phage minor tail protein L	-
JUD77_RS06485	1295170..1295919	+	750	WP_112102023.1	C40 family peptidase	-
JUD77_RS06490	1295856..1296470	+	615	WP_112072211.1	tail assembly protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	kdsA / licD / licC / licB / licA / htrB / rfaE	1256924..1360609	103685

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-81)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14868.05 Da
• Isoelectric Point: 4.4987

>T38444 WP_015701504.1 NZ_AP024414:1291922-1292305 [Haemophilus influenzae]
MKQEWEVILQDPLLNWLETLAEDDVLKIYAALELLSTEGPQLSRPYADTLQGSKYTNLKELRVQSKLSVFRLFYIFDPVR
QAIVLCGGDKKGKKEKLFYKEMIALAEQTYDDYLSELTKEQENEREI

Nucleotide

• Length: 384 bp

>T38444 NZ_AP024414:1291922-1292305 [Haemophilus influenzae]
ATGAAACAAGAATGGGAAGTAATTTTACAAGACCCACTTTTGAACTGGTTAGAAACGCTGGCAGAAGATGACGTATTAAA
AATCTATGCGGCGTTGGAATTATTATCAACAGAAGGTCCACAATTAAGCAGACCTTATGCGGATACGCTGCAAGGCTCTA
AATATACCAATTTAAAAGAATTGCGAGTACAGTCTAAATTATCGGTATTCCGTTTATTTTATATTTTCGACCCTGTCAGA
CAAGCGATTGTTTTATGTGGCGGAGATAAAAAAGGCAAGAAAGAAAAACTCTTTTACAAAGAGATGATTGCCCTAGCGGA
ACAAACCTATGATGATTACCTTTCTGAATTAACTAAGGAGCAAGAAAATGAGCGTGAAATTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11258.21 Da
• Isoelectric Point: 9.5655

>AT38444 WP_005651224.1 NZ_AP024414:1292289-1292591 [Haemophilus influenzae]
MSVKFKDLMNNLPAEKQAKVKAMADDMRMELQLYRIREELELSQKQMAEALSISQPSVVALEKRGNDIKLSSVKRYIEAM
GGVLNLSVELPTGKTVTFNL

Nucleotide

• Length: 303 bp

>AT38444 NZ_AP024414:1292289-1292591 [Haemophilus influenzae]
ATGAGCGTGAAATTTAAAGATCTGATGAATAATCTTCCAGCTGAAAAGCAAGCGAAAGTGAAAGCTATGGCAGACGATAT
GCGAATGGAGTTACAACTTTACCGTATTCGTGAAGAATTAGAGCTTTCACAAAAGCAAATGGCAGAAGCATTAAGTATTT
CTCAGCCGTCAGTTGTCGCCCTTGAAAAACGTGGCAATGACATTAAATTATCATCAGTTAAACGTTACATTGAAGCAATG
GGTGGTGTGTTAAATTTATCGGTTGAATTACCCACAGGAAAAACAGTTACTTTCAACTTATAG

Structures

Toxin

• AlphaFold DB: A0A2R3G0F4

Antitoxin

• AlphaFold DB: A0A502K5Z3