Detailed information of TA system TA038428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1019944..1020770
• Replicon: chromosome
• Accession: NZ_AP024354
• Organism: Pseudomonas alcaligenes strain KAM426

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: JUH08_RS04755
• Protein ID: WP_088177213.1
• Coordinates: 1019944..1020447 (-)
• Length: 168 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: JUH08_RS04760
• Protein ID: WP_088177212.1
• Coordinates: 1020444..1020770 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JUH08_RS04725	1015978..1016247	+	270	WP_203790994.1	hypothetical protein	-
JUH08_RS04730	1016244..1016804	+	561	WP_203790996.1	zeta toxin family protein	-
JUH08_RS04735	1016816..1017978	+	1163	WP_128688142.1	IS3 family transposase	-
JUH08_RS04740	1018262..1018822	-	561	WP_203790998.1	hypothetical protein	-
JUH08_RS04745	1018908..1019123	-	216	WP_203791000.1	hypothetical protein	-
JUH08_RS04750	1019395..1019601	+	207	WP_203791002.1	hypothetical protein	-
JUH08_RS04755	1019944..1020447	-	504	WP_088177213.1	type II toxin-antitoxin system YhaV family toxin	Toxin
JUH08_RS04760	1020444..1020770	-	327	WP_088177212.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
JUH08_RS04765	1021026..1022351	+	1326	WP_088177214.1	HipA domain-containing protein	-
JUH08_RS04770	1022352..1022618	+	267	WP_088177211.1	helix-turn-helix domain-containing protein	-
JUH08_RS04775	1022666..1022869	-	204	WP_088177210.1	hypothetical protein	-
JUH08_RS04780	1022955..1023086	-	132	WP_143492042.1	DUF4113 domain-containing protein	-
JUH08_RS04785	1023650..1024630	-	981	Protein_937	IS5-like element ISPa16 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	xcpA/pilD / pilC / pilB	947520..1076676	129156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (16-152)

Antitoxin

• PrlF antitoxin (8-106)

Sequences

Toxin

Protein

• Length: 168 a.a.
• Molecular weight: 19179.83 Da
• Isoelectric Point: 9.9994

>T38428 WP_088177213.1 NZ_AP024354:c1020447-1019944 [Pseudomonas alcaligenes]
MSHDHGKPMVAHGWTVFAHPLFLDQLESLIQKVELSKQKDPVGYRKKNAAKRLAAIAKLAFDVIPQDPSKPEYRQGGTLG
EEHKHWFRAKFFQQYRLFFRYHGPSKIIVLAWVNDDDTKRAYESSDDAYKVFRKMLASGHPPDAWDKLLKEATQENARLN
SAATSVN

Nucleotide

• Length: 504 bp

>T38428 NZ_AP024354:c1020447-1019944 [Pseudomonas alcaligenes]
ATGAGCCACGACCACGGCAAGCCAATGGTTGCCCATGGCTGGACAGTATTTGCGCACCCCTTGTTTCTGGATCAGCTCGA
AAGCCTCATTCAGAAAGTTGAGCTCTCAAAACAAAAAGACCCCGTGGGCTATCGCAAGAAGAATGCAGCCAAGCGGCTGG
CAGCCATCGCCAAGCTGGCTTTTGACGTTATCCCACAAGACCCGAGCAAGCCCGAATACCGCCAAGGTGGCACATTGGGC
GAGGAACATAAGCACTGGTTCCGTGCCAAGTTTTTTCAGCAGTATCGGCTGTTCTTCCGGTACCACGGCCCAAGCAAGAT
AATCGTCTTGGCGTGGGTTAATGATGACGACACCAAGCGTGCCTATGAGAGCAGCGATGACGCCTACAAGGTGTTTCGAA
AAATGCTCGCGAGCGGACATCCGCCTGACGCGTGGGATAAGCTGCTGAAGGAAGCCACGCAGGAAAACGCACGATTAAAT
TCGGCCGCCACCTCGGTCAACTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12030.33 Da
• Isoelectric Point: 4.5700

>AT38428 WP_088177212.1 NZ_AP024354:c1020770-1020444 [Pseudomonas alcaligenes]
MARALEVESTLTDRYQTTVPETVRRALQLNKRDKIHYSIRPSGEVVLSRAEHSEDDPAIGQFLSFLAHDIAANPQHLQAI
DSNLVARIHSLVDSNEIDLDAPLSADDE

Nucleotide

• Length: 327 bp

>AT38428 NZ_AP024354:c1020770-1020444 [Pseudomonas alcaligenes]
ATGGCCAGAGCACTCGAAGTCGAATCCACCCTTACCGACCGCTACCAGACGACGGTACCAGAGACTGTTCGCCGCGCACT
TCAGCTAAACAAGCGCGACAAAATTCATTACTCGATTCGCCCCAGCGGTGAGGTCGTACTGAGCCGTGCAGAGCACTCTG
AGGATGACCCGGCAATCGGCCAGTTTCTCAGTTTTCTTGCACATGACATCGCAGCGAACCCTCAGCACCTGCAAGCCATA
GATTCAAATCTCGTGGCACGCATCCACTCGCTGGTCGATAGCAATGAGATAGATCTGGATGCCCCGCTGTCGGCAGACGA
TGAATGA