Detailed information of TA system TA038374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2077834..2078396
• Replicon: chromosome
• Accession: NZ_AP024329
• Organism: Erwinia rhapontici strain MAFF 311153

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KJS81_RS09735
• Protein ID: WP_171148675.1
• Coordinates: 2077834..2078112 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KJS81_RS09740
• Protein ID: WP_159337278.1
• Coordinates: 2078112..2078396 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KJS81_RS09710 (ERHA53_19210)	2073695..2074249	-	555	WP_133843312.1	LuxR C-terminal-related transcriptional regulator	-
KJS81_RS09715 (ERHA53_19220)	2074541..2075287	+	747	WP_133843314.1	winged helix-turn-helix domain-containing protein	-
KJS81_RS09720 (ERHA53_19230)	2075284..2075754	+	471	WP_133843316.1	hypothetical protein	-
KJS81_RS09725 (ERHA53_19240)	2075968..2076387	+	420	WP_133843318.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
KJS81_RS09730 (ERHA53_19250)	2076390..2077652	+	1263	WP_171148676.1	translesion error-prone DNA polymerase V subunit UmuC	-
KJS81_RS09735 (ERHA53_19260)	2077834..2078112	+	279	WP_171148675.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KJS81_RS09740 (ERHA53_19270)	2078112..2078396	+	285	WP_159337278.1	HigA family addiction module antitoxin	Antitoxin
KJS81_RS09745 (ERHA53_19280)	2078431..2078577	-	147	WP_159337277.1	hypothetical protein	-
KJS81_RS09750 (ERHA53_19290)	2078852..2079232	+	381	WP_133843352.1	putative holin	-
KJS81_RS09755 (ERHA53_19300)	2079219..2079506	+	288	WP_133843354.1	phage holin family protein	-
KJS81_RS09760 (ERHA53_19310)	2079686..2081437	+	1752	WP_212814177.1	glycosyl hydrolase family 28-related protein	-
KJS81_RS09765 (ERHA53_19320)	2081738..2081938	+	201	WP_133843358.1	hypothetical protein	-
KJS81_RS09770 (ERHA53_19330)	2081950..2082198	-	249	WP_133843360.1	hypothetical protein	-
KJS81_RS09775 (ERHA53_19340)	2082465..2082710	-	246	WP_133843362.1	DinI-like family protein	-
KJS81_RS09780 (ERHA53_19350)	2082865..2083089	+	225	WP_133843364.1	YgdI/YgdR family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2073695..2085595	11900

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (10-67)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10539.09 Da
• Isoelectric Point: 7.3176

>T38374 WP_171148675.1 NZ_AP024329:2077834-2078112 [Erwinia rhapontici]
MIKSFKHKGLKKFFLKDDSSGIEQKLVGRIRNRLSVIDAAEKIGAIDMPGYDLHPLKGDRQEMWSVSVSGNWRITFTFID
GDAEILNLEDYH

Nucleotide

• Length: 279 bp

>T38374 NZ_AP024329:2077834-2078112 [Erwinia rhapontici]
ATGATTAAAAGCTTTAAGCATAAGGGACTAAAAAAATTCTTTCTAAAAGATGATAGTAGCGGTATCGAACAAAAGCTGGT
GGGTCGAATAAGGAACCGCCTGAGCGTGATTGATGCGGCAGAAAAAATCGGAGCGATCGATATGCCTGGTTACGACCTGC
ATCCGCTCAAAGGTGATCGGCAGGAAATGTGGTCAGTTAGCGTATCGGGTAACTGGCGTATCACGTTTACGTTTATTGAT
GGCGATGCTGAGATTCTTAACCTGGAGGATTACCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10374.99 Da
• Isoelectric Point: 9.8926

>AT38374 WP_159337278.1 NZ_AP024329:2078112-2078396 [Erwinia rhapontici]
MKMANPAHPGEIIAEALDDMRVSVRQFARAMEIAPSTASRLLSGYTAVTPEMALRLAIVVGSTAETWMKIQTNYSLHQAR
QTLDTRHLHRLSAA

Nucleotide

• Length: 285 bp

>AT38374 NZ_AP024329:2078112-2078396 [Erwinia rhapontici]
ATGAAAATGGCTAACCCTGCTCACCCTGGCGAGATCATTGCAGAAGCGCTGGATGATATGCGCGTTAGCGTCCGCCAGTT
CGCCCGGGCAATGGAAATTGCACCCTCAACGGCCAGCCGGTTGTTGTCAGGATATACGGCGGTAACACCTGAGATGGCGT
TGCGTCTCGCTATCGTTGTCGGGAGTACGGCGGAAACCTGGATGAAAATACAGACAAATTACAGCCTGCATCAGGCACGT
CAAACGCTGGACACCCGTCATCTGCATCGACTATCGGCAGCCTGA