Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3811142..3811799 Replicon chromosome
Accession NZ_AP024281
Organism Enterobacter asburiae strain Ent261

Toxin (Protein)


Gene name higB Uniprot ID A0A808IF03
Locus tag R1N_RS18520 Protein ID WP_033146338.1
Coordinates 3811142..3811441 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A808ICP3
Locus tag R1N_RS18525 Protein ID WP_033146339.1
Coordinates 3811452..3811799 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
R1N_RS18510 (R1N_36370) 3807510..3809708 + 2199 WP_033146337.1 type I secretion system permease/ATPase -
R1N_RS18515 (R1N_36380) 3809719..3810888 + 1170 WP_086374946.1 HlyD family efflux transporter periplasmic adaptor subunit -
R1N_RS18520 3811142..3811441 + 300 WP_033146338.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
R1N_RS18525 (R1N_36390) 3811452..3811799 + 348 WP_033146339.1 HigA family addiction module antitoxin Antitoxin
R1N_RS18530 (R1N_36400) 3811844..3812269 + 426 WP_033146340.1 nucleoside triphosphatase NudI -
R1N_RS18535 (R1N_36410) 3812241..3812972 - 732 WP_033146341.1 helix-turn-helix transcriptional regulator -
R1N_RS18540 (R1N_36430) 3813070..3813489 + 420 WP_033146342.1 nuclear transport factor 2 family protein -
R1N_RS18545 (R1N_36440) 3813492..3814010 - 519 WP_033146343.1 hypothetical protein -
R1N_RS18550 (R1N_36450) 3814095..3815276 - 1182 WP_033146344.1 PLP-dependent aminotransferase family protein -
R1N_RS18555 (R1N_36460) 3815296..3816183 - 888 WP_033146345.1 LysR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-95)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(8-86)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11691.32 Da        Isoelectric Point: 10.0346

>T38305 WP_033146338.1 NZ_AP024281:3811142-3811441 [Enterobacter asburiae]
MISYFRDQWLEDFFLYGRSSNVIPANLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
TEGKAEDLYLSPHKYTQHK

Download         Length: 300 bp

>T38305 NZ_AP024281:3811142-3811441 [Enterobacter asburiae]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATTCCTGCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGCAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGATATTCCTCAATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
ACAGAGGGTAAAGCAGAAGATCTCTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13066.02 Da        Isoelectric Point: 7.0134

>AT38305 WP_033146339.1 NZ_AP024281:3811452-3811799 [Enterobacter asburiae]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNPRTQEELSRIKTVADVMAKRKSGQPDVA

Download         Length: 348 bp

>AT38305 NZ_AP024281:3811452-3811799 [Enterobacter asburiae]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAATAATCGCAAGCTGA
CTGCCGATATGGCGATCAAACTGGCAAAAGCCTTTGATACCACGATTGAATTTTGGCTTAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCCAACCCCAGGACGCAGGAGGAGCTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAGCCGGATGTAGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A808IF03

Antitoxin

Source ID Structure
AlphaFold DB A0A808ICP3

References