Detailed information of TA system TA038304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3133395..3134055
• Replicon: chromosome
• Accession: NZ_AP024281
• Organism: Enterobacter asburiae strain Ent261

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: R1N_RS15295
• Protein ID: WP_033145931.1
• Coordinates: 3133702..3134055 (-)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MX79
• Locus tag: R1N_RS15290
• Protein ID: WP_029741460.1
• Coordinates: 3133395..3133697 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
R1N_RS15260 (R1N_30090)	3129304..3130665	+	1362	WP_003036316.1	HEPN/Toprim-associated domain-containing protein	-
R1N_RS15265	3130835..3131029	+	195	WP_080277779.1	hypothetical protein	-
R1N_RS15270 (R1N_30100)	3130986..3131318	-	333	WP_033145927.1	type II toxin-antitoxin system PemK/MazF family toxin	-
R1N_RS15275 (R1N_30110)	3131319..3131576	-	258	WP_033145928.1	antitoxin	-
R1N_RS15280 (R1N_30120)	3131868..3132257	-	390	WP_033145929.1	RidA family protein	-
R1N_RS15285 (R1N_30130)	3132399..3133319	+	921	WP_033145930.1	LysR family transcriptional regulator	-
R1N_RS15290 (R1N_30140)	3133395..3133697	-	303	WP_029741460.1	XRE family transcriptional regulator	Antitoxin
R1N_RS15295 (R1N_30150)	3133702..3134055	-	354	WP_033145931.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
R1N_RS15300 (R1N_30160)	3134233..3135126	-	894	WP_033145932.1	LysR substrate-binding domain-containing protein	-
R1N_RS15305 (R1N_30170)	3135297..3135905	+	609	WP_080277780.1	glutathione S-transferase family protein	-
R1N_RS15310 (R1N_30180)	3135949..3136899	-	951	WP_023336219.1	HTH-type transcriptional regulator Cbl	-
R1N_RS15315 (R1N_30190)	3136996..3137913	-	918	WP_033145933.1	nitrogen assimilation transcriptional regulator NAC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13577.40 Da
• Isoelectric Point: 8.0361

>T38304 WP_033145931.1 NZ_AP024281:c3134055-3133702 [Enterobacter asburiae]
VWAINTTDRFDRWFTSLDDTDRASVLAALLVLREKGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
MLCAGNKVGNERRFYDEMLLVADQEFTHWLDRLKERE

Nucleotide

• Length: 354 bp

>T38304 NZ_AP024281:c3134055-3133702 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGTACTGGCATC
ATGCTTTGTGCTGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCAGGAATTCAC
TCACTGGCTGGATAGATTAAAAGAGAGGGAATAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11050.83 Da
• Isoelectric Point: 5.7316

>AT38304 WP_029741460.1 NZ_AP024281:c3133697-3133395 [Enterobacter asburiae]
MGRTLEQLIADEKPEVVASAQAIATDILLNIHLAELREKVQKTQVEMAQALGIRQPTVAVMEKPGRDLKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Nucleotide

• Length: 303 bp

>AT38304 NZ_AP024281:c3133697-3133395 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGTGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGACGGCTCGCACTATGAGTTTGTGCTGTAG

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MX79