Detailed information of TA system TA038166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3748127..3748821
• Replicon: chromosome
• Accession: NZ_AP024205
• Organism: Escherichia coli strain TA8571

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JG732_RS18210
• Protein ID: WP_001263493.1
• Coordinates: 3748127..3748525 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JG732_RS18215
• Protein ID: WP_000554757.1
• Coordinates: 3748528..3748821 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JG732_RS18180	3743127..3744371	-	1245	WP_000189541.1	esterase FrsA	-
-	3743787..3743867	-	81	NuclAT_9	-	-
-	3743787..3743867	-	81	NuclAT_9	-	-
-	3743787..3743867	-	81	NuclAT_9	-	-
-	3743787..3743867	-	81	NuclAT_9	-	-
JG732_RS18185	3744463..3744921	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JG732_RS18190	3745182..3746639	+	1458	WP_063084593.1	cytosol nonspecific dipeptidase	-
JG732_RS18195	3746696..3747217	-	522	Protein_3568	peptide chain release factor H	-
JG732_RS18200	3747216..3747419	-	204	Protein_3569	RNA ligase RtcB family protein	-
JG732_RS18205	3747665..3748117	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JG732_RS18210	3748127..3748525	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JG732_RS18215	3748528..3748821	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JG732_RS18220	3748873..3749928	-	1056	WP_063084594.1	DNA polymerase IV	-
JG732_RS18225	3749999..3750922	-	924	WP_001232547.1	putative lateral flagellar export/assembly protein LafU	-
JG732_RS18230	3750925..3751788	-	864	WP_001169518.1	flagellar motor stator protein MotA	-
JG732_RS18235	3751801..3752517	-	717	WP_000938723.1	FliA/WhiG family RNA polymerase sigma factor	-
JG732_RS18240	3752537..3753004	-	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T38166 WP_001263493.1 NZ_AP024205:c3748525-3748127 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T38166 NZ_AP024205:c3748525-3748127 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT38166 WP_000554757.1 NZ_AP024205:c3748821-3748528 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT38166 NZ_AP024205:c3748821-3748528 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6