Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1860487..1860989 Replicon chromosome
Accession NZ_AP024205
Organism Escherichia coli strain TA8571

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JG732_RS08955 Protein ID WP_000767829.1
Coordinates 1860735..1860989 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JG732_RS08950 Protein ID WP_001259255.1
Coordinates 1860487..1860738 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JG732_RS08925 1855665..1856732 - 1068 WP_000080141.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JG732_RS08930 1856732..1857802 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
JG732_RS08935 1857799..1859103 - 1305 WP_001367667.1 histidinol dehydrogenase -
JG732_RS08940 1859109..1860008 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
JG732_RS08945 1860154..1860204 - 51 WP_001364200.1 his operon leader peptide -
JG732_RS08950 1860487..1860738 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JG732_RS08955 1860735..1860989 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JG732_RS08960 1861072..1861896 + 825 WP_000754737.1 SDR family oxidoreductase -
JG732_RS08965 1861942..1862871 + 930 WP_000803351.1 LysR family transcriptional regulator -
JG732_RS08970 1863086..1863148 + 63 WP_010723108.1 membrane protein YoeI -
JG732_RS08975 1863138..1864496 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JG732_RS08980 1864675..1865733 + 1059 WP_000492316.1 transport protein YeeE -
JG732_RS08985 1865747..1865974 + 228 WP_000985278.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T38154 WP_000767829.1 NZ_AP024205:1860735-1860989 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T38154 NZ_AP024205:1860735-1860989 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT38154 WP_001259255.1 NZ_AP024205:1860487-1860738 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT38154 NZ_AP024205:1860487-1860738 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References