Detailed information of TA system TA038061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 117565..118217
• Replicon: plasmid pM311693
• Accession: NZ_AP024162
• Organism: Ralstonia solanacearum strain MAFF 311693

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7I8FRY7
• Locus tag: JL096_RS17845
• Protein ID: WP_071092328.1
• Coordinates: 117565..117909 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JL096_RS17850
• Protein ID: WP_020425351.1
• Coordinates: 117906..118217 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JL096_RS17830	113261..114424	-	1164	WP_201016583.1	2-methylcitrate synthase	-
JL096_RS17835	114476..115372	-	897	WP_197368739.1	methylisocitrate lyase	-
JL096_RS17840	115565..117514	+	1950	WP_197368738.1	propionate catabolism operon regulatory protein PrpR	-
JL096_RS17845	117565..117909	+	345	WP_071092328.1	toxin	Toxin
JL096_RS17850	117906..118217	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
JL096_RS17855	118357..119166	-	810	WP_064478250.1	DUF2242 domain-containing protein	-
JL096_RS17860	120411..122249	+	1839	WP_197368737.1	AAA family ATPase	-
JL096_RS17865	122439..122768	+	330	WP_028861316.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cyaB / adeG / acrB / fliI / fliG / fliM / flgG / flgC / cheY / cheB / cheW / motA	1..2117975	2117975

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13222.98 Da
• Isoelectric Point: 8.0098

>T38061 WP_071092328.1 NZ_AP024162:117565-117909 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDECKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T38061 NZ_AP024162:117565-117909 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAACAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTTACTACCACTGGGATGGAGGCGGCCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGTGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT38061 WP_020425351.1 NZ_AP024162:117906-118217 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT38061 NZ_AP024162:117906-118217 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A7I8FRY7