Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3931113..3931654 Replicon chromosome
Accession NZ_AP024130
Organism Escherichia coli strain SI-NP020

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag FFK26_RS19005 Protein ID WP_000615976.1
Coordinates 3931376..3931654 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FFK26_RS19000 Protein ID WP_000729704.1
Coordinates 3931113..3931373 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFK26_RS18975 (3926896) 3926896..3927681 - 786 WP_000207589.1 putative lateral flagellar export/assembly protein LafU -
FFK26_RS18980 (3927653) 3927653..3929365 + 1713 Protein_3727 flagellar biosynthesis protein FlhA -
FFK26_RS18985 (3929470) 3929470..3929748 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FFK26_RS18990 (3929741) 3929741..3930097 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FFK26_RS18995 (3930154) 3930154..3930927 - 774 WP_000543899.1 C40 family peptidase -
FFK26_RS19000 (3931113) 3931113..3931373 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FFK26_RS19005 (3931376) 3931376..3931654 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FFK26_RS19010 (3931810) 3931810..3932550 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FFK26_RS19015 (3932521) 3932521..3933288 - 768 WP_000333380.1 class II glutamine amidotransferase -
FFK26_RS19020 (3933494) 3933494..3934072 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T38035 WP_000615976.1 NZ_AP024130:3931376-3931654 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T38035 NZ_AP024130:3931376-3931654 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT38035 WP_000729704.1 NZ_AP024130:3931113-3931373 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT38035 NZ_AP024130:3931113-3931373 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References