Detailed information of TA system TA038007

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5243783..5244385
• Replicon: chromosome
• Accession: NZ_AP024126
• Organism: Escherichia coli strain NS-NP030

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FFJ14_RS25500
• Protein ID: WP_000897305.1
• Coordinates: 5243783..5244094 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFJ14_RS25505
• Protein ID: WP_000356397.1
• Coordinates: 5244095..5244385 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFJ14_RS25470 (5238813)	5238813..5239598	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
FFJ14_RS25475 (5239697)	5239697..5240296	+	600	WP_001295269.1	glucose-1-phosphatase	-
FFJ14_RS25480 (5240290)	5240290..5241162	+	873	WP_000920762.1	virulence factor BrkB family protein	-
FFJ14_RS25485 (5241159)	5241159..5241596	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
FFJ14_RS25490 (5241641)	5241641..5242582	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FFJ14_RS25495 (5242646)	5242646..5243554	-	909	WP_001162704.1	alpha/beta hydrolase	-
FFJ14_RS25500 (5243783)	5243783..5244094	+	312	WP_000897305.1	hypothetical protein	Toxin
FFJ14_RS25505 (5244095)	5244095..5244385	+	291	WP_000356397.1	NadS family protein	Antitoxin
FFJ14_RS25510 (5244971)	5244971..5245189	+	219	WP_001315930.1	CopG family transcriptional regulator	-
FFJ14_RS25515 (5245409)	5245409..5245651	+	243	WP_001087409.1	protein YiiF	-
FFJ14_RS25520 (5245981)	5245981..5246910	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FFJ14_RS25525 (5246907)	5246907..5247542	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FFJ14_RS25530 (5247539)	5247539..5248441	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T38007 WP_000897305.1 NZ_AP024126:5243783-5244094 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T38007 NZ_AP024126:5243783-5244094 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT38007 WP_000356397.1 NZ_AP024126:5244095-5244385 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT38007 NZ_AP024126:5244095-5244385 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7