Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 880470..881011 Replicon chromosome
Accession NZ_AP024126
Organism Escherichia coli strain NS-NP030

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag FFJ14_RS04145 Protein ID WP_000615982.1
Coordinates 880470..880748 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FFJ14_RS04150 Protein ID WP_000729704.1
Coordinates 880751..881011 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFJ14_RS04130 (878052) 878052..878630 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FFJ14_RS04135 (878836) 878836..879603 + 768 WP_000333380.1 class II glutamine amidotransferase -
FFJ14_RS04140 (879574) 879574..880314 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FFJ14_RS04145 (880470) 880470..880748 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FFJ14_RS04150 (880751) 880751..881011 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FFJ14_RS04155 (881197) 881197..881970 + 774 WP_000543899.1 C40 family peptidase -
FFJ14_RS04160 (882027) 882027..882383 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FFJ14_RS04165 (882376) 882376..882654 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FFJ14_RS04170 (882759) 882759..884471 - 1713 Protein_813 flagellar biosynthesis protein FlhA -
FFJ14_RS04175 (884443) 884443..885228 + 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T37976 WP_000615982.1 NZ_AP024126:c880748-880470 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T37976 NZ_AP024126:c880748-880470 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT37976 WP_000729704.1 NZ_AP024126:c881011-880751 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT37976 NZ_AP024126:c881011-880751 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References