Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4382569..4383110 Replicon chromosome
Accession NZ_AP024123
Organism Escherichia coli strain KS-NP019

Toxin (Protein)


Gene name yafQ Uniprot ID E9TC49
Locus tag FFG67_RS21035 Protein ID WP_000615982.1
Coordinates 4382569..4382847 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag FFG67_RS21040 Protein ID WP_000729704.1
Coordinates 4382850..4383110 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFG67_RS21020 (4380151) 4380151..4380729 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
FFG67_RS21025 (4380935) 4380935..4381702 + 768 WP_000333380.1 class II glutamine amidotransferase -
FFG67_RS21030 (4381673) 4381673..4382413 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
FFG67_RS21035 (4382569) 4382569..4382847 - 279 WP_000615982.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
FFG67_RS21040 (4382850) 4382850..4383110 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
FFG67_RS21045 (4383296) 4383296..4384069 + 774 WP_000543899.1 C40 family peptidase -
FFG67_RS21050 (4384126) 4384126..4384482 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
FFG67_RS21055 (4384475) 4384475..4384753 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
FFG67_RS21060 (4384858) 4384858..4386570 - 1713 Protein_4116 flagellar biosynthesis protein FlhA -
FFG67_RS21065 (4386542) 4386542..4387327 + 786 WP_000207567.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10816.59 Da        Isoelectric Point: 10.0702

>T37963 WP_000615982.1 NZ_AP024123:c4382847-4382569 [Escherichia coli]
MIQRDIEYSGQFSKDVKVAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T37963 NZ_AP024123:c4382847-4382569 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT37963 WP_000729704.1 NZ_AP024123:c4383110-4382850 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT37963 NZ_AP024123:c4383110-4382850 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References