Detailed information of TA system TA037960

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3501117..3501719
• Replicon: chromosome
• Accession: NZ_AP024123
• Organism: Escherichia coli strain KS-NP019

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FFG67_RS16930
• Protein ID: WP_000897305.1
• Coordinates: 3501117..3501428 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFG67_RS16935
• Protein ID: WP_000356397.1
• Coordinates: 3501429..3501719 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFG67_RS16900 (3496147)	3496147..3496932	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
FFG67_RS16905 (3497031)	3497031..3497630	+	600	WP_001295269.1	glucose-1-phosphatase	-
FFG67_RS16910 (3497624)	3497624..3498496	+	873	WP_000920762.1	virulence factor BrkB family protein	-
FFG67_RS16915 (3498493)	3498493..3498930	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
FFG67_RS16920 (3498975)	3498975..3499916	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FFG67_RS16925 (3499980)	3499980..3500888	-	909	WP_001162704.1	alpha/beta hydrolase	-
FFG67_RS16930 (3501117)	3501117..3501428	+	312	WP_000897305.1	hypothetical protein	Toxin
FFG67_RS16935 (3501429)	3501429..3501719	+	291	WP_000356397.1	NadS family protein	Antitoxin
FFG67_RS16940 (3502305)	3502305..3502523	+	219	WP_001315930.1	CopG family transcriptional regulator	-
FFG67_RS16945 (3502743)	3502743..3502985	+	243	WP_001087409.1	protein YiiF	-
FFG67_RS16950 (3503315)	3503315..3504244	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FFG67_RS16955 (3504241)	3504241..3504876	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FFG67_RS16960 (3504873)	3504873..3505775	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T37960 WP_000897305.1 NZ_AP024123:3501117-3501428 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T37960 NZ_AP024123:3501117-3501428 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT37960 WP_000356397.1 NZ_AP024123:3501429-3501719 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT37960 NZ_AP024123:3501429-3501719 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7