Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 94245..94498 Replicon plasmid JML285_P2
Accession NZ_AP024116
Organism Escherichia coli strain JML285

Toxin (Protein)


Gene name srnB Uniprot ID G9G1E3
Locus tag FFF71_RS25125 Protein ID WP_001312851.1
Coordinates 94349..94498 (+) Length 50 a.a.

Antitoxin (RNA)


Gene name srnC
Locus tag -
Coordinates 94245..94301 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FFF71_RS25095 (90771) 90771..91517 + 747 WP_040084630.1 conjugal transfer pilus acetylase TraX -
FFF71_RS25100 (91572) 91572..92132 + 561 WP_040084627.1 fertility inhibition protein FinO -
FFF71_RS25105 (92268) 92268..92480 + 213 WP_040084624.1 hypothetical protein -
FFF71_RS25110 (92726) 92726..93187 + 462 WP_040084621.1 thermonuclease family protein -
FFF71_RS25115 (93256) 93256..93441 + 186 WP_040084637.1 Hha/YmoA family nucleoid-associated regulatory protein -
FFF71_RS25120 (93480) 93480..94070 + 591 WP_040084619.1 DUF2726 domain-containing protein -
- (94245) 94245..94301 - 57 NuclAT_0 - Antitoxin
- (94245) 94245..94301 - 57 NuclAT_0 - Antitoxin
- (94245) 94245..94301 - 57 NuclAT_0 - Antitoxin
- (94245) 94245..94301 - 57 NuclAT_0 - Antitoxin
FFF71_RS25125 (94349) 94349..94498 + 150 WP_001312851.1 Hok/Gef family protein Toxin
FFF71_RS25130 (94782) 94782..95039 + 258 WP_000083826.1 replication regulatory protein RepA -
FFF71_RS26040 (95143) 95143..95327 + 185 Protein_119 plasmid copy control protein CopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(A) / tet(M) - 1..95339 95339


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(4-45)


Sequences

Toxin        


Download         Length: 50 a.a.        Molecular weight: 5542.67 Da        Isoelectric Point: 8.7678

>T37933 WP_001312851.1 NZ_AP024116:94349-94498 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Download         Length: 150 bp

>T37933 NZ_AP024116:94349-94498 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 57 bp

>AT37933 NZ_AP024116:c94301-94245 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C8K5

Antitoxin

Download structure file

References