Detailed information of TA system TA037926

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4795982..4796584
• Replicon: chromosome
• Accession: NZ_AP024114
• Organism: Escherichia coli strain JML285

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FFF71_RS22980
• Protein ID: WP_000897305.1
• Coordinates: 4796273..4796584 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FFF71_RS22975
• Protein ID: WP_000356397.1
• Coordinates: 4795982..4796272 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFF71_RS22945 (4791118)	4791118..4791753	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FFF71_RS22950 (4791750)	4791750..4792679	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
FFF71_RS22960 (4794117)	4794117..4794488	+	372	Protein_4498	IS110 family transposase	-
FFF71_RS22965 (4794717)	4794717..4794959	-	243	WP_137515171.1	protein YiiF	-
FFF71_RS22970 (4795178)	4795178..4795396	-	219	WP_001295676.1	CopG family transcriptional regulator	-
FFF71_RS22975 (4795982)	4795982..4796272	-	291	WP_000356397.1	NadS family protein	Antitoxin
FFF71_RS22980 (4796273)	4796273..4796584	-	312	WP_000897305.1	hypothetical protein	Toxin
FFF71_RS22985 (4796813)	4796813..4797721	+	909	WP_001299484.1	alpha/beta hydrolase	-
FFF71_RS22990 (4797785)	4797785..4798726	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FFF71_RS22995 (4798771)	4798771..4799208	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
FFF71_RS23000 (4799205)	4799205..4800077	-	873	WP_000920762.1	virulence factor BrkB family protein	-
FFF71_RS23005 (4800071)	4800071..4800670	-	600	WP_001295269.1	glucose-1-phosphatase	-
FFF71_RS23010 (4800769)	4800769..4801554	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T37926 WP_000897305.1 NZ_AP024114:c4796584-4796273 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T37926 NZ_AP024114:c4796584-4796273 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTTCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT37926 WP_000356397.1 NZ_AP024114:c4796272-4795982 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT37926 NZ_AP024114:c4796272-4795982 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7