Detailed information of TA system TA037903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 599251..600050
• Replicon: chromosome
• Accession: NZ_AP024114
• Organism: Escherichia coli strain JML285

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: -
• Locus tag: FFF71_RS02940
• Protein ID: WP_000347253.1
• Coordinates: 599251..599715 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: -
• Locus tag: FFF71_RS02945
• Protein ID: WP_137515341.1
• Coordinates: 599715..600050 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FFF71_RS02910 (594252)	594252..594686	-	435	WP_000948824.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
FFF71_RS02915 (594704)	594704..595582	-	879	WP_001295548.1	PTS N-acetylgalactosamine transporter subunit IID	-
FFF71_RS02920 (595572)	595572..596351	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
FFF71_RS02925 (596362)	596362..596835	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
FFF71_RS02930 (596858)	596858..598138	-	1281	WP_000681910.1	tagatose-bisphosphate aldolase subunit KbaZ	-
FFF71_RS02935 (598387)	598387..599196	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
FFF71_RS02940 (599251)	599251..599715	-	465	WP_000347253.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
FFF71_RS02945 (599715)	599715..600050	-	336	WP_137515341.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
FFF71_RS02950 (600199)	600199..601770	-	1572	WP_001273766.1	galactarate dehydratase	-
FFF71_RS02955 (602145)	602145..603479	+	1335	WP_137515340.1	galactarate/glucarate/glycerate transporter GarP	-
FFF71_RS02960 (603495)	603495..604265	+	771	WP_001058209.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	599251..611038	11787

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17764.14 Da
• Isoelectric Point: 9.4947

>T37903 WP_000347253.1 NZ_AP024114:c599715-599251 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEETH

Nucleotide

• Length: 465 bp

>T37903 NZ_AP024114:c599715-599251 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12361.90 Da
• Isoelectric Point: 4.8616

>AT37903 WP_137515341.1 NZ_AP024114:c600050-599715 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
SQKNRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT37903 NZ_AP024114:c600050-599715 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCAGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
TCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTACCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACGATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
TCGCAAAAAAATCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA