Detailed information of TA system TA037895

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89040..89461
• Replicon: plasmid ECSC054_P1
• Accession: NZ_AP024113
• Organism: Escherichia coli strain ECSC054

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: FE985_RS25750
• Protein ID: WP_096937776.1
• Coordinates: 89040..89165 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	89263..89461 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE985_RS25710 (84116)	84116..84343	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
FE985_RS25715 (84437)	84437..85123	-	687	WP_001563714.1	PAS domain-containing protein	-
FE985_RS25720 (85313)	85313..85696	-	384	WP_001563713.1	conjugal transfer relaxosome DNA-binding protein TraM	-
FE985_RS25725 (86030)	86030..86620	+	591	WP_001375132.1	transglycosylase SLT domain-containing protein	-
FE985_RS25730 (86916)	86916..87737	-	822	WP_137445206.1	DUF932 domain-containing protein	-
FE985_RS25735 (87856)	87856..88142	-	287	Protein_104	hypothetical protein	-
FE985_RS25740 (88167)	88167..88373	-	207	WP_000547965.1	hypothetical protein	-
FE985_RS25745 (88443)	88443..88739	+	297	Protein_106	hypothetical protein	-
FE985_RS25750 (89040)	89040..89165	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
FE985_RS26230 (89107)	89107..89256	-	150	Protein_108	DUF5431 family protein	-
- (89263)	89263..89461	-	199	NuclAT_0	-	Antitoxin
- (89263)	89263..89461	-	199	NuclAT_0	-	Antitoxin
- (89263)	89263..89461	-	199	NuclAT_0	-	Antitoxin
- (89263)	89263..89461	-	199	NuclAT_0	-	Antitoxin
FE985_RS25755 (89430)	89430..90192	-	763	Protein_109	plasmid SOS inhibition protein A	-
FE985_RS25760 (90189)	90189..90623	-	435	WP_000845928.1	conjugation system SOS inhibitor PsiB	-
FE985_RS25765 (90678)	90678..91517	-	840	Protein_111	hypothetical protein	-
FE985_RS25770 (91712)	91712..93253	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
FE985_RS25775 (93268)	93268..94014	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	iucA / iucB / iucC / iucD / iutA	1..126311	126311

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T37895 WP_096937776.1 NZ_AP024113:c89165-89040 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T37895 NZ_AP024113:c89165-89040 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT37895 NZ_AP024113:c89461-89263 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin