Detailed information of TA system TA037891

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5102779..5103381
• Replicon: chromosome
• Accession: NZ_AP024112
• Organism: Escherichia coli strain ECSC054

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: FE985_RS24275
• Protein ID: WP_000897305.1
• Coordinates: 5103070..5103381 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FE985_RS24270
• Protein ID: WP_000356395.1
• Coordinates: 5102779..5103069 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FE985_RS24235 (5098525)	5098525..5099160	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
FE985_RS24240 (5099157)	5099157..5100086	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
FE985_RS24245 (5100268)	5100268..5100510	-	243	WP_001309881.1	CopG family transcriptional regulator	-
FE985_RS24250 (5100729)	5100729..5100947	-	219	WP_001295676.1	CopG family transcriptional regulator	-
FE985_RS24260 (5102143)	5102143..5102421	-	279	WP_001315112.1	hypothetical protein	-
FE985_RS24265 (5102473)	5102473..5102694	-	222	WP_001550354.1	hypothetical protein	-
FE985_RS24270 (5102779)	5102779..5103069	-	291	WP_000356395.1	NadS family protein	Antitoxin
FE985_RS24275 (5103070)	5103070..5103381	-	312	WP_000897305.1	hypothetical protein	Toxin
FE985_RS24280 (5103610)	5103610..5104518	+	909	WP_001550353.1	alpha/beta hydrolase	-
FE985_RS26190 (5104686)	5104686..5105600	-	915	WP_109553727.1	transposase	-
FE985_RS26195 (5105613)	5105613..5106500	-	888	Protein_4769	hypothetical protein	-
FE985_RS24290 (5106916)	5106916..5107857	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
FE985_RS24295 (5107902)	5107902..5108339	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5101001..5101294	293

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T37891 WP_000897305.1 NZ_AP024112:c5103381-5103070 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T37891 NZ_AP024112:c5103381-5103070 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT37891 WP_000356395.1 NZ_AP024112:c5103069-5102779 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT37891 NZ_AP024112:c5103069-5102779 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7