Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2064713..2065215 Replicon chromosome
Accession NZ_AP024112
Organism Escherichia coli strain ECSC054

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FE985_RS09830 Protein ID WP_000767829.1
Coordinates 2064961..2065215 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FE985_RS09825 Protein ID WP_001259255.1
Coordinates 2064713..2064964 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FE985_RS09800 (2059891) 2059891..2060958 - 1068 WP_000080068.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FE985_RS09805 (2060958) 2060958..2062028 - 1071 WP_001099222.1 histidinol-phosphate transaminase -
FE985_RS09810 (2062025) 2062025..2063329 - 1305 WP_001468360.1 histidinol dehydrogenase -
FE985_RS09815 (2063335) 2063335..2064234 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FE985_RS09820 (2064380) 2064380..2064430 - 51 WP_001364200.1 his operon leader peptide -
FE985_RS09825 (2064713) 2064713..2064964 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FE985_RS09830 (2064961) 2064961..2065215 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FE985_RS09835 (2065298) 2065298..2066122 + 825 WP_000754734.1 SDR family oxidoreductase -
FE985_RS09840 (2066168) 2066168..2067097 + 930 WP_000803351.1 LysR substrate-binding domain-containing protein -
FE985_RS09845 (2067312) 2067312..2067374 + 63 WP_010723108.1 membrane protein YoeI -
FE985_RS09850 (2067364) 2067364..2068722 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FE985_RS09855 (2068901) 2068901..2069959 + 1059 WP_000492321.1 thiosulfate utilization transporter TsuA/YeeE -
FE985_RS09860 (2069973) 2069973..2070200 + 228 WP_000985267.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T37870 WP_000767829.1 NZ_AP024112:2064961-2065215 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T37870 NZ_AP024112:2064961-2065215 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT37870 WP_001259255.1 NZ_AP024112:2064713-2064964 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT37870 NZ_AP024112:2064713-2064964 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References