Detailed information of TA system TA037858

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/MqsA(antitoxin)
• Location: 120936..121588
• Replicon: plasmid pMAFF241648
• Accession: NZ_AP024108
• Organism: Ralstonia solanacearum strain MAFF 241648

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7I8FRY7
• Locus tag: JK148_RS18290
• Protein ID: WP_071092328.1
• Coordinates: 120936..121280 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JK148_RS18295
• Protein ID: WP_020425351.1
• Coordinates: 121277..121588 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JK148_RS18275	116632..117795	-	1164	WP_016726526.1	2-methylcitrate synthase	-
JK148_RS18280	117847..118743	-	897	WP_197368739.1	methylisocitrate lyase	-
JK148_RS18285	118936..120885	+	1950	WP_197368738.1	propionate catabolism operon regulatory protein PrpR	-
JK148_RS18290	120936..121280	+	345	WP_071092328.1	toxin	Toxin
JK148_RS18295	121277..121588	+	312	WP_020425351.1	transcriptional regulator	Antitoxin
JK148_RS18300	121728..122537	-	810	WP_064478250.1	DUF2242 domain-containing protein	-
JK148_RS18305	123782..125620	+	1839	WP_197368737.1	AAA family ATPase	-
JK148_RS18310	125810..126139	+	330	WP_028861316.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	motA / cheW / cheB / cheY / adeG / acrB / fliI / fliG / fliM / flgG / flgC / cyaB	1..2107950	2107950

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (41-91)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13222.98 Da
• Isoelectric Point: 8.0098

>T37858 WP_071092328.1 NZ_AP024108:120936-121280 [Ralstonia solanacearum]
MDATFIELPPFQRLREHYLSDDEYRALQQELLARPDAGDVIKGTGGLRKLRFSDKRRGKGKRGGLRVIYYHWDGGGQFWM
FVVYDKGEADDLSSDECKVLARLLEQEVKARSER

Nucleotide

• Length: 345 bp

>T37858 NZ_AP024108:120936-121280 [Ralstonia solanacearum]
ATGGATGCTACCTTTATCGAGTTGCCGCCTTTTCAGAGGCTGCGGGAGCACTATCTCTCGGACGACGAGTACCGAGCGCT
CCAACAGGAACTGCTGGCCCGGCCGGACGCCGGCGACGTCATCAAGGGGACAGGTGGCCTACGCAAGCTGCGATTTTCGG
ACAAGCGGCGCGGCAAAGGTAAGCGCGGCGGTCTGCGCGTGATTTACTACCACTGGGATGGAGGCGGCCAATTCTGGATG
TTTGTCGTCTACGACAAGGGCGAGGCGGACGACCTTTCCAGCGACGAGTGCAAAGTGCTGGCACGGCTGTTGGAGCAGGA
AGTCAAGGCAAGGAGTGAGCGATGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11619.65 Da
• Isoelectric Point: 10.7132

>AT37858 WP_020425351.1 NZ_AP024108:121277-121588 [Ralstonia solanacearum]
MKRNLFAELKEGMDALAAEREGKMTLRKVKVRVLQPLDVTAEEIKAVRAAAHASQAVMARRLRVNVRTYQNWEQGTAKPN
TQAAVLIKLVEKHPETLQMLEAL

Nucleotide

• Length: 312 bp

>AT37858 NZ_AP024108:121277-121588 [Ralstonia solanacearum]
ATGAAACGGAACCTGTTTGCTGAGTTGAAGGAGGGGATGGATGCGCTTGCCGCAGAGCGCGAAGGAAAGATGACCCTTCG
CAAGGTGAAGGTCCGCGTGCTCCAGCCGCTTGACGTGACAGCCGAAGAAATCAAGGCCGTTCGCGCAGCGGCACATGCTT
CACAGGCTGTCATGGCACGTCGACTGCGCGTCAATGTGCGCACCTACCAGAACTGGGAACAAGGAACCGCCAAACCGAAC
ACCCAGGCGGCTGTGTTGATCAAGCTCGTCGAGAAGCATCCGGAAACGTTGCAGATGCTGGAAGCGCTTTAA

Structures

Toxin

• AlphaFold DB: A0A7I8FRY7