Detailed information of TA system TA037818

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 52830..53100
• Replicon: plasmid pM941-NDM5
• Accession: NZ_AP023454
• Organism: Klebsiella pneumoniae strain M941

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: JLF44_RS26730
• Protein ID: WP_001312861.1
• Coordinates: 52942..53100 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	52830..52893 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JLF44_RS26705	48541..49068	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
JLF44_RS26710	49126..49359	+	234	WP_000006003.1	DUF905 family protein	-
JLF44_RS26715	49420..51443	+	2024	Protein_61	ParB/RepB/Spo0J family partition protein	-
JLF44_RS26720	51512..51946	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
JLF44_RS26725	51943..52662	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	52674..52898	+	225	NuclAT_0	-	-
-	52674..52898	+	225	NuclAT_0	-	-
-	52674..52898	+	225	NuclAT_0	-	-
-	52674..52898	+	225	NuclAT_0	-	-
-	52830..52893	-	64	-	-	Antitoxin
JLF44_RS26730	52942..53100	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
JLF44_RS26735	53338..53715	-	378	Protein_65	hypothetical protein	-
JLF44_RS26740	54015..54311	+	297	WP_001272251.1	hypothetical protein	-
JLF44_RS26745	54422..55243	+	822	WP_001234445.1	DUF945 domain-containing protein	-
JLF44_RS26750	55540..56142	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
JLF44_RS26755	56465..56848	+	384	WP_001354030.1	relaxosome protein TraM	-
JLF44_RS26760	57042..57713	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
JLF44_RS26765	57850..58077	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..93470	93470

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T37818 WP_001312861.1 NZ_AP023454:52942-53100 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T37818 NZ_AP023454:52942-53100 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT37818 NZ_AP023454:c52893-52830 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin