Detailed information of TA system TA037787

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2058815..2059549
• Replicon: chromosome
• Accession: NZ_AP023452
• Organism: Citrobacter koseri strain MPUCK001

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A8AI82
• Locus tag: MPUCK001_RS09535
• Protein ID: WP_012132927.1
• Coordinates: 2058815..2059120 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A8AI81
• Locus tag: MPUCK001_RS09540
• Protein ID: WP_012132926.1
• Coordinates: 2059208..2059549 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MPUCK001_RS09510	2054148..2054867	+	720	WP_104868817.1	aspartate/glutamate racemase family protein	-
MPUCK001_RS09515	2054896..2055903	+	1008	WP_049009338.1	ABC transporter substrate-binding protein	-
MPUCK001_RS09520	2055900..2056670	+	771	WP_058668982.1	ABC transporter permease	-
MPUCK001_RS09525	2056670..2057446	+	777	WP_047457671.1	ABC transporter ATP-binding protein	-
MPUCK001_RS09530	2057480..2058586	-	1107	WP_104868816.1	4Fe-4S binding protein	-
MPUCK001_RS09535	2058815..2059120	+	306	WP_012132927.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MPUCK001_RS09540	2059208..2059549	+	342	WP_012132926.1	HigA family addiction module antidote protein	Antitoxin
MPUCK001_RS09545	2059689..2059994	-	306	WP_047457663.1	chaperone modulator CbpM	-
MPUCK001_RS09550	2059994..2060914	-	921	WP_190286989.1	curved DNA-binding protein	-
MPUCK001_RS09555	2061144..2061509	+	366	WP_047457658.1	hypothetical protein	-
MPUCK001_RS09560	2061558..2063444	+	1887	WP_104868815.1	thioredoxin family protein	-
MPUCK001_RS09565	2063441..2064064	+	624	WP_104868814.1	thioredoxin domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (7-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-69)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11972.70 Da
• Isoelectric Point: 8.3565

>T37787 WP_012132927.1 NZ_AP023452:2058815-2059120 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKIPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Nucleotide

• Length: 306 bp

>T37787 NZ_AP023452:2058815-2059120 [Citrobacter koseri]
ATGCCAAAGAAACTTGTCAGTATTGAAGACTTCCGTGACCAGTGGCTGGATGATTTTTTTATGTACTCTGCACCACATAA
GAAAATTCCCCCGGATATACATTCAGCATTGGCCCGTAAGCTCGATATCATAAACGCGGCAGTGAGCTACAAGGATTTGA
AGTCACCGCCGGGTAACAGATACGAAGAACTGGAAGGTAAATTGCAGGAATATTCTTCGATAAGAGTTAACAAGCAATAT
CGTTTGATATTCAAATGGGTGAACGGCAAGGCACAAGACCTCTACCTTGATCCACATGAGTATTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13062.91 Da
• Isoelectric Point: 5.1864

>AT37787 WP_012132926.1 NZ_AP023452:2059208-2059549 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERQKQEKKVA

Nucleotide

• Length: 342 bp

>AT37787 NZ_AP023452:2059208-2059549 [Citrobacter koseri]
ATGAACCAGGCAACCAGAAAACCCACGACAGTTGGTGATATCCTTCTGTATGAATACCTGGAACCGCTTGATCTCAAAAT
TAATGATCTCGCGGAAATGTTGCAGGTGCATCGTAATACCGCCAGCGCATTGGTTAACAACAATCGTAAACTCACGACTG
ACATGGCTTTTCGCCTGTCTAAAGTTTTCGATACCTCTGTAGAGTTCTGGATGAACCTGCAAACTGCTGTAGATGTATGG
GAAACTGAAAACAATGCCCGCCTTCAGGTAGAGATGAGCAGGGTGATTACGGCGAGCGATTTTCTTGCGGAACGTCAGAA
ACAAGAAAAGAAAGTCGCGTGA

Structures

Toxin

• AlphaFold DB: A8AI82

Antitoxin

• AlphaFold DB: A8AI81