37787

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2058815..2059549 Replicon chromosome
Accession NZ_AP023452
Organism Citrobacter koseri strain MPUCK001

Toxin (Protein)


Gene name higB Uniprot ID A8AI82
Locus tag MPUCK001_RS09535 Protein ID WP_012132927.1
Coordinates 2058815..2059120 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A8AI81
Locus tag MPUCK001_RS09540 Protein ID WP_012132926.1
Coordinates 2059208..2059549 (+) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MPUCK001_RS09510 2054148..2054867 + 720 WP_104868817.1 aspartate/glutamate racemase family protein -
MPUCK001_RS09515 2054896..2055903 + 1008 WP_049009338.1 ABC transporter substrate-binding protein -
MPUCK001_RS09520 2055900..2056670 + 771 WP_058668982.1 ABC transporter permease -
MPUCK001_RS09525 2056670..2057446 + 777 WP_047457671.1 ABC transporter ATP-binding protein -
MPUCK001_RS09530 2057480..2058586 - 1107 WP_104868816.1 4Fe-4S binding protein -
MPUCK001_RS09535 2058815..2059120 + 306 WP_012132927.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MPUCK001_RS09540 2059208..2059549 + 342 WP_012132926.1 HigA family addiction module antidote protein Antitoxin
MPUCK001_RS09545 2059689..2059994 - 306 WP_047457663.1 chaperone modulator CbpM -
MPUCK001_RS09550 2059994..2060914 - 921 WP_190286989.1 curved DNA-binding protein -
MPUCK001_RS09555 2061144..2061509 + 366 WP_047457658.1 hypothetical protein -
MPUCK001_RS09560 2061558..2063444 + 1887 WP_104868815.1 thioredoxin family protein -
MPUCK001_RS09565 2063441..2064064 + 624 WP_104868814.1 thioredoxin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-101)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-69)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11972.70 Da        Isoelectric Point: 8.3565

>T37787 WP_012132927.1 NZ_AP023452:2058815-2059120 [Citrobacter koseri]
MPKKLVSIEDFRDQWLDDFFMYSAPHKKIPPDIHSALARKLDIINAAVSYKDLKSPPGNRYEELEGKLQEYSSIRVNKQY
RLIFKWVNGKAQDLYLDPHEY

Download         Length: 306 bp

>T37787 NZ_AP023452:2058815-2059120 [Citrobacter koseri]
ATGCCAAAGAAACTTGTCAGTATTGAAGACTTCCGTGACCAGTGGCTGGATGATTTTTTTATGTACTCTGCACCACATAA
GAAAATTCCCCCGGATATACATTCAGCATTGGCCCGTAAGCTCGATATCATAAACGCGGCAGTGAGCTACAAGGATTTGA
AGTCACCGCCGGGTAACAGATACGAAGAACTGGAAGGTAAATTGCAGGAATATTCTTCGATAAGAGTTAACAAGCAATAT
CGTTTGATATTCAAATGGGTGAACGGCAAGGCACAAGACCTCTACCTTGATCCACATGAGTATTAA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13062.91 Da        Isoelectric Point: 5.1864

>AT37787 WP_012132926.1 NZ_AP023452:2059208-2059549 [Citrobacter koseri]
MNQATRKPTTVGDILLYEYLEPLDLKINDLAEMLQVHRNTASALVNNNRKLTTDMAFRLSKVFDTSVEFWMNLQTAVDVW
ETENNARLQVEMSRVITASDFLAERQKQEKKVA

Download         Length: 342 bp

>AT37787 NZ_AP023452:2059208-2059549 [Citrobacter koseri]
ATGAACCAGGCAACCAGAAAACCCACGACAGTTGGTGATATCCTTCTGTATGAATACCTGGAACCGCTTGATCTCAAAAT
TAATGATCTCGCGGAAATGTTGCAGGTGCATCGTAATACCGCCAGCGCATTGGTTAACAACAATCGTAAACTCACGACTG
ACATGGCTTTTCGCCTGTCTAAAGTTTTCGATACCTCTGTAGAGTTCTGGATGAACCTGCAAACTGCTGTAGATGTATGG
GAAACTGAAAACAATGCCCGCCTTCAGGTAGAGATGAGCAGGGTGATTACGGCGAGCGATTTTCTTGCGGAACGTCAGAA
ACAAGAAAAGAAAGTCGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A8AI82

Antitoxin

Source ID Structure
AlphaFold DB A8AI81

References