Detailed information of TA system TA037782

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 127117..127844
• Replicon: plasmid pN260-2
• Accession: NZ_AP023449
• Organism: Enterobacter roggenkampii strain OIPH-N260

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A663AUB1
• Locus tag: OIPHN260_RS25670
• Protein ID: WP_000558568.1
• Coordinates: 127117..127428 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A7U3M0L1
• Locus tag: OIPHN260_RS25675
• Protein ID: WP_072199090.1
• Coordinates: 127425..127844 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
OIPHN260_RS25645	122901..124646	+	1746	WP_004187005.1	ATP-grasp domain-containing protein	-
OIPHN260_RS25650	124959..125039	-	81	Protein_101	IS1 family transposase	-
OIPHN260_RS25655	125090..126058	-	969	WP_074132802.1	IS5 family transposase	-
OIPHN260_RS25660	126081..126476	+	396	Protein_103	lactate utilization protein C	-
OIPHN260_RS25665	126526..126906	-	381	Protein_104	gluconate permease	-
OIPHN260_RS25670	127117..127428	+	312	WP_000558568.1	type II toxin-antitoxin system HigB family toxin	Toxin
OIPHN260_RS25675	127425..127844	+	420	WP_072199090.1	helix-turn-helix domain-containing protein	Antitoxin
OIPHN260_RS25680	127881..128090	-	210	WP_020806042.1	hypothetical protein	-
OIPHN260_RS25685	128664..129669	-	1006	Protein_108	IS110-like element IS4321 family transposase	-
OIPHN260_RS25690	129807..130766	-	960	WP_023294863.1	cation diffusion facilitator family transporter	-
OIPHN260_RS25695	130786..131352	-	567	WP_011638900.1	recombinase family protein	-
OIPHN260_RS25700	131360..131656	-	297	WP_025714488.1	zinc ribbon domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mcr-10	mrkA / mrkB / mrkC / mrkF / mrkJ	1..244996	244996
	-	inside	IScluster/Tn	-	-	125090..140372	15282

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12372.11 Da
• Isoelectric Point: 10.0935

>T37782 WP_000558568.1 NZ_AP023449:127117-127428 [Enterobacter roggenkampii]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T37782 NZ_AP023449:127117-127428 [Enterobacter roggenkampii]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15592.75 Da
• Isoelectric Point: 4.4075

>AT37782 WP_072199090.1 NZ_AP023449:127425-127844 [Enterobacter roggenkampii]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIEALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT37782 NZ_AP023449:127425-127844 [Enterobacter roggenkampii]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGAAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A663AUB1

Antitoxin

• AlphaFold DB: A0A7U3M0L1