Detailed information of TA system TA037744

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53966..54235
• Replicon: plasmid pM71901
• Accession: NZ_AP023434
• Organism: Escherichia coli strain M719

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: M2043_RS23800
• Protein ID: WP_001372321.1
• Coordinates: 54110..54235 (+)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53966..54031 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M2043_RS23765	49676..50203	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
M2043_RS23770	50261..50494	+	234	WP_000006003.1	DUF905 family protein	-
M2043_RS23775	50555..52578	+	2024	Protein_65	ParB/RepB/Spo0J family partition protein	-
M2043_RS23780	52647..53081	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
M2043_RS23785	53078..53840	+	763	Protein_67	plasmid SOS inhibition protein A	-
-	53809..54033	+	225	NuclAT_0	-	-
-	53809..54033	+	225	NuclAT_0	-	-
-	53809..54033	+	225	NuclAT_0	-	-
-	53809..54033	+	225	NuclAT_0	-	-
M2043_RS23790	53818..53997	-	180	WP_001309233.1	hypothetical protein	-
-	53966..54031	+	66	-	-	Antitoxin
M2043_RS23795	54019..54168	+	150	Protein_69	plasmid maintenance protein Mok	-
M2043_RS23800	54110..54235	+	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
M2043_RS23805	54554..54850	-	297	Protein_71	hypothetical protein	-
M2043_RS23810	55150..55446	+	297	WP_001272251.1	hypothetical protein	-
M2043_RS23815	55557..56378	+	822	WP_001234445.1	DUF932 domain-containing protein	-
M2043_RS23820	56675..57277	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
M2043_RS23825	57600..57983	+	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
M2043_RS23830	58177..58848	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
M2043_RS23835	58985..59212	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T37744 WP_001372321.1 NZ_AP023434:54110-54235 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T37744 NZ_AP023434:54110-54235 [Escherichia coli]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT37744 NZ_AP023434:53966-54031 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Antitoxin