37724

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 1088298..1088992 Replicon chromosome
Accession NZ_AP023433
Organism Escherichia coli strain M719

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag M2043_RS05245 Protein ID WP_001263489.1
Coordinates 1088594..1088992 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag M2043_RS05240 Protein ID WP_000554758.1
Coordinates 1088298..1088591 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2043_RS05220 (1083930) 1083930..1084427 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M2043_RS05225 (1084651) 1084651..1086363 - 1713 Protein_1008 flagellar biosynthesis protein FlhA -
M2043_RS05230 (1086335) 1086335..1087120 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
M2043_RS05235 (1087191) 1087191..1088246 + 1056 WP_001226164.1 DNA polymerase IV -
M2043_RS05240 (1088298) 1088298..1088591 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
M2043_RS05245 (1088594) 1088594..1088992 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
M2043_RS05250 (1089002) 1089002..1089454 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
M2043_RS05255 (1089772) 1089772..1089978 + 207 Protein_1014 RtcB family protein -
M2043_RS05260 (1089974) 1089974..1090495 + 522 Protein_1015 peptide chain release factor H -
M2043_RS05265 (1090552) 1090552..1092009 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
M2043_RS05270 (1092270) 1092270..1092728 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
- (1093324) 1093324..1093404 + 81 NuclAT_12 - -
- (1093324) 1093324..1093404 + 81 NuclAT_12 - -
- (1093324) 1093324..1093404 + 81 NuclAT_12 - -
- (1093324) 1093324..1093404 + 81 NuclAT_12 - -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catA1 / qepA1 / blaOXA-1 / ant(3'')-Ia gmhA/lpcA 1072211..1123879 51668


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T37724 WP_001263489.1 NZ_AP023433:1088594-1088992 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T37724 NZ_AP023433:1088594-1088992 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT37724 WP_000554758.1 NZ_AP023433:1088298-1088591 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT37724 NZ_AP023433:1088298-1088591 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References