Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1082254..1082795 Replicon chromosome
Accession NZ_AP023433
Organism Escherichia coli strain M719

Toxin (Protein)


Gene name yafQ Uniprot ID Q47149
Locus tag M2043_RS05205 Protein ID WP_000615983.1
Coordinates 1082254..1082532 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q47150
Locus tag M2043_RS05210 Protein ID WP_000729703.1
Coordinates 1082535..1082795 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M2043_RS05190 (1079836) 1079836..1080414 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
M2043_RS05195 (1080620) 1080620..1081387 + 768 WP_000333380.1 class II glutamine amidotransferase -
M2043_RS05200 (1081358) 1081358..1082098 - 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
M2043_RS05205 (1082254) 1082254..1082532 - 279 WP_000615983.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
M2043_RS05210 (1082535) 1082535..1082795 - 261 WP_000729703.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
M2043_RS05215 (1083005) 1083005..1083754 + 750 WP_000056849.1 C40 family peptidase -
M2043_RS05220 (1083930) 1083930..1084427 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
M2043_RS05225 (1084651) 1084651..1086363 - 1713 Protein_1008 flagellar biosynthesis protein FlhA -
M2043_RS05230 (1086335) 1086335..1087120 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catA1 / qepA1 / blaOXA-1 / ant(3'')-Ia gmhA/lpcA 1072211..1123879 51668


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(4-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-85)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10846.62 Da        Isoelectric Point: 9.9941

>T37723 WP_000615983.1 NZ_AP023433:c1082532-1082254 [Escherichia coli]
MIQRDIEYSGQYSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T37723 NZ_AP023433:c1082532-1082254 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9405.80 Da        Isoelectric Point: 5.0758

>AT37723 WP_000729703.1 NZ_AP023433:c1082795-1082535 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT37723 NZ_AP023433:c1082795-1082535 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4Q2U

Antitoxin

Source ID Structure
PDB 4Q2U
AlphaFold DB A0A5F1F716

References