Detailed information of TA system TA037716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3592578..3593272
• Replicon: chromosome
• Accession: NZ_AP023427
• Organism: Escherichia coli strain HUE1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: IF662_RS17220
• Protein ID: WP_001263493.1
• Coordinates: 3592578..3592976 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: IF662_RS17225
• Protein ID: WP_000554757.1
• Coordinates: 3592979..3593272 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IF662_RS17190	3587578..3588822	-	1245	WP_000189541.1	esterase FrsA	-
-	3588238..3588318	-	81	NuclAT_11	-	-
-	3588238..3588318	-	81	NuclAT_11	-	-
-	3588238..3588318	-	81	NuclAT_11	-	-
-	3588238..3588318	-	81	NuclAT_11	-	-
IF662_RS17195	3588914..3589372	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
IF662_RS17200	3589633..3591090	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
IF662_RS17205	3591147..3591668	-	522	Protein_3361	peptide chain release factor H	-
IF662_RS17210	3591667..3591870	-	204	Protein_3362	RNA ligase RtcB family protein	-
IF662_RS17215	3592116..3592568	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
IF662_RS17220	3592578..3592976	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
IF662_RS17225	3592979..3593272	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
IF662_RS17230	3593324..3594379	-	1056	WP_063501914.1	DNA polymerase IV	-
IF662_RS17235	3594450..3595235	-	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
IF662_RS17240	3595207..3596919	+	1713	Protein_3368	flagellar biosynthesis protein FlhA	-
IF662_RS17245	3597143..3597640	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T37716 WP_001263493.1 NZ_AP023427:c3592976-3592578 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T37716 NZ_AP023427:c3592976-3592578 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT37716 WP_000554757.1 NZ_AP023427:c3593272-3592979 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT37716 NZ_AP023427:c3593272-3592979 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6