Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1776793..1777295 Replicon chromosome
Accession NZ_AP023427
Organism Escherichia coli strain HUE1

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag IF662_RS08365 Protein ID WP_000767829.1
Coordinates 1777041..1777295 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag IF662_RS08360 Protein ID WP_001259255.1
Coordinates 1776793..1777044 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IF662_RS08335 1771974..1773041 - 1068 WP_001572812.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
IF662_RS08340 1773038..1774108 - 1071 WP_001572811.1 histidinol-phosphate transaminase -
IF662_RS08345 1774105..1775409 - 1305 WP_001572810.1 histidinol dehydrogenase -
IF662_RS08350 1775415..1776314 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
IF662_RS08355 1776460..1776510 - 51 WP_001364200.1 his operon leader peptide -
IF662_RS08360 1776793..1777044 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
IF662_RS08365 1777041..1777295 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
IF662_RS08370 1777378..1778202 + 825 WP_000754737.1 SDR family oxidoreductase -
IF662_RS08375 1778248..1779177 + 930 WP_000803360.1 LysR family transcriptional regulator -
IF662_RS08380 1779392..1779454 + 63 WP_010723108.1 membrane protein YoeI -
IF662_RS08385 1779444..1780802 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
IF662_RS08390 1780981..1782039 + 1059 WP_000492340.1 transport protein YeeE -
IF662_RS08395 1782053..1782280 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T37703 WP_000767829.1 NZ_AP023427:1777041-1777295 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T37703 NZ_AP023427:1777041-1777295 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT37703 WP_001259255.1 NZ_AP023427:1776793-1777044 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT37703 NZ_AP023427:1776793-1777044 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References