Detailed information of TA system TA037662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3641806..3642500
• Replicon: chromosome
• Accession: NZ_AP023358
• Organism: Micromonospora endophytica strain NBRC 109090

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: -
• Locus tag: Jiend_RS17160
• Protein ID: WP_223256759.1
• Coordinates: 3641806..3642195 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: HigA2
• Uniprot ID: -
• Locus tag: Jiend_RS17165
• Protein ID: WP_212808287.1
• Coordinates: 3642228..3642500 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Jiend_RS17135 (Jiend_35520)	3637388..3637882	-	495	WP_111244308.1	YajQ family cyclic di-GMP-binding protein	-
Jiend_RS17145 (Jiend_35530)	3638419..3639750	+	1332	WP_223256756.1	tyrosine-type recombinase/integrase	-
Jiend_RS17150 (Jiend_35540)	3639786..3640337	+	552	WP_146603519.1	hypothetical protein	-
Jiend_RS17155 (Jiend_35550)	3640975..3641706	+	732	WP_119663995.1	hypothetical protein	-
Jiend_RS17160 (Jiend_35560)	3641806..3642195	+	390	WP_223256759.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Jiend_RS17165 (Jiend_35570)	3642228..3642500	+	273	WP_212808287.1	helix-turn-helix transcriptional regulator	Antitoxin
Jiend_RS17170 (Jiend_35580)	3642536..3643261	-	726	WP_249412178.1	protein phosphatase 2C domain-containing protein	-
Jiend_RS31355 (Jiend_35590)	3643342..3644184	-	843	WP_223256757.1	hypothetical protein	-
Jiend_RS31360 (Jiend_35600)	3644296..3644616	+	321	WP_223256758.1	hypothetical protein	-
Jiend_RS17185 (Jiend_35610)	3644702..3644947	+	246	WP_111244298.1	precorrin-4 C11-methyltransferase	-
Jiend_RS17190 (Jiend_35620)	3645188..3645631	-	444	WP_111244297.1	hypothetical protein	-
Jiend_RS32140	3645956..3646090	+	135	WP_258378301.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (25-118)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-79)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14366.19 Da
• Isoelectric Point: 4.9839

>T37662 WP_223256759.1 NZ_AP023358:3641806-3642195 [Micromonospora endophytica]
VDYSGGAGEWRIDATDELDEWFVRLSKEDPDSANQVAAAIDILAREGPALGRPLVDRVKGSDYKNMKELRPGSSGGTEIR
ILFAFDPTRTAILLVAGNKAGNWKGWYEMNILEADRLFAEHLRTMKGQR

Nucleotide

• Length: 390 bp

>T37662 NZ_AP023358:3641806-3642195 [Micromonospora endophytica]
GTGGACTACAGTGGGGGCGCAGGCGAGTGGCGCATCGACGCCACCGACGAGTTGGACGAGTGGTTCGTTCGGCTGAGCAA
GGAAGACCCAGACTCTGCCAACCAGGTAGCCGCCGCCATCGACATCCTCGCCAGGGAGGGCCCAGCCTTGGGGCGTCCAC
TGGTCGACAGGGTCAAGGGCTCGGACTACAAGAACATGAAGGAACTCCGGCCCGGCTCCTCGGGTGGGACCGAGATCCGC
ATCCTGTTTGCTTTCGACCCGACCCGGACAGCCATCCTGCTGGTGGCAGGGAACAAGGCCGGCAACTGGAAGGGCTGGTA
CGAGATGAACATTCTCGAAGCTGACAGGCTCTTCGCCGAACACCTTCGAACGATGAAGGGACAACGCTGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10213.72 Da
• Isoelectric Point: 5.9224

>AT37662 WP_212808287.1 NZ_AP023358:3642228-3642500 [Micromonospora endophytica]
MNLDEAKVAEHKERMLAEVRATRLREMRERRGLTQREMAERMQISQPRVAAIEKGEVPATEVGTIERYVAALGGKLEIIA
DFDGDRFALS

Nucleotide

• Length: 273 bp

>AT37662 NZ_AP023358:3642228-3642500 [Micromonospora endophytica]
ATGAATCTCGATGAGGCCAAGGTCGCCGAGCACAAGGAGCGCATGCTCGCTGAGGTCCGGGCCACCCGCCTGCGTGAGAT
GCGCGAGCGTCGAGGTCTGACCCAACGAGAGATGGCCGAGCGCATGCAGATATCCCAGCCGAGGGTTGCCGCGATTGAGA
AGGGTGAGGTGCCGGCGACGGAGGTCGGCACCATCGAGCGGTACGTCGCTGCTCTCGGTGGCAAGCTGGAGATCATCGCC
GACTTTGATGGTGACCGGTTCGCCCTGAGCTGA