Detailed information of TA system TA037656

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 7027528..7028237
• Replicon: chromosome
• Accession: NZ_AP023354
• Organism: Actinocatenispora sera strain NBRC 101916

Toxin (Protein)

• Gene name: HigB2
• Uniprot ID: A0A810LC71
• Locus tag: Asera_RS32790
• Protein ID: WP_030445057.1
• Coordinates: 7027528..7027926 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: Asera_RS32795
• Protein ID: WP_030445056.1
• Coordinates: 7027923..7028237 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Asera_RS32770 (Asera_65890)	7022822..7023343	+	522	WP_084131012.1	SigE family RNA polymerase sigma factor	-
Asera_RS32775 (Asera_65900)	7023347..7024351	+	1005	WP_030445060.1	CU044_5270 family protein	-
Asera_RS32780 (Asera_65910)	7024525..7025748	+	1224	WP_157034670.1	hypothetical protein	-
Asera_RS32785 (Asera_65920)	7025921..7027492	+	1572	WP_030445058.1	NAD(P)/FAD-dependent oxidoreductase	-
Asera_RS32790 (Asera_65930)	7027528..7027926	+	399	WP_030445057.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Asera_RS32795 (Asera_65940)	7027923..7028237	+	315	WP_030445056.1	helix-turn-helix transcriptional regulator	Antitoxin
Asera_RS32800 (Asera_65950)	7028547..7029338	+	792	WP_030445055.1	rRNA adenine N-6-methyltransferase family protein	-
Asera_RS32805 (Asera_65960)	7029426..7031807	+	2382	WP_030445054.1	phosphoketolase family protein	-
Asera_RS32810 (Asera_65970)	7031809..7032900	+	1092	WP_030445053.1	acetate/propionate family kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (19-116)

Antitoxin

• HigA2-like, helix-turn-helix domain (18-92)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14872.88 Da
• Isoelectric Point: 8.5366

>T37656 WP_030445057.1 NZ_AP023354:7027528-7027926 [Actinocatenispora sera]
MSTNGERWTIRVTSEVRAWLRELNTAQPSTYRDVNAAIDLLAEIGPALGRPLVDTLHGSDVHNLKELRPRSGREMALRIL
FAFDPWSQAILLTAGNKAGNWSAWYTEAIPVAEKAYAAWLIAEAQRRKETDR

Nucleotide

• Length: 399 bp

>T37656 NZ_AP023354:7027528-7027926 [Actinocatenispora sera]
ATGTCCACCAACGGGGAAAGATGGACCATCCGGGTCACTAGCGAGGTTCGCGCGTGGCTTCGTGAACTCAATACAGCCCA
GCCCAGCACCTACCGAGACGTCAACGCAGCCATCGACCTGCTTGCGGAAATCGGTCCGGCACTGGGCCGGCCACTGGTCG
ACACGCTGCATGGATCCGACGTACACAACCTGAAAGAACTGCGCCCCCGATCGGGCAGGGAGATGGCACTGCGAATTCTG
TTCGCGTTCGACCCTTGGTCGCAGGCGATTCTGCTGACCGCCGGCAACAAGGCGGGTAACTGGTCAGCCTGGTACACCGA
GGCGATCCCGGTCGCCGAGAAGGCATATGCGGCTTGGCTGATCGCGGAAGCTCAGCGCCGGAAGGAGACGGATCGATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11699.99 Da
• Isoelectric Point: 4.3759

>AT37656 WP_030445056.1 NZ_AP023354:7027923-7028237 [Actinocatenispora sera]
MTEFYDWDDVRAELHDGDDEALAQARARTEAWISAYHLAEERKRLGLTQRDVAEAMGVTPGRVSQIENGDLDVNEVATLG
RYAQALGAKLRIIFDYGTDMRQIA

Nucleotide

• Length: 315 bp

>AT37656 NZ_AP023354:7027923-7028237 [Actinocatenispora sera]
ATGACCGAGTTCTACGACTGGGACGACGTTCGGGCCGAGTTGCACGACGGCGATGACGAGGCGCTGGCGCAGGCGCGGGC
GCGCACCGAGGCGTGGATCAGCGCGTATCACCTCGCGGAGGAGCGGAAGCGACTCGGGTTGACGCAGCGGGATGTTGCCG
AGGCCATGGGCGTCACACCAGGGCGAGTCAGTCAGATCGAGAACGGCGACCTCGACGTCAACGAGGTAGCCACGCTCGGT
CGCTATGCACAGGCGCTGGGCGCGAAACTGCGGATCATCTTCGACTACGGGACGGACATGCGGCAGATCGCCTGA

Structures

Toxin

• AlphaFold DB: A0A810LC71