Detailed information of TA system TA037649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4880213..4880817
• Replicon: chromosome
• Accession: NZ_AP023348
• Organism: Pseudomonas sp. RtIB026

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PRtIB026_RS21995
• Protein ID: WP_203647823.1
• Coordinates: 4880213..4880533 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PRtIB026_RS22000
• Protein ID: WP_203647824.1
• Coordinates: 4880530..4880817 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PRtIB026_RS21985	4876465..4878009	-	1545	WP_203647822.1	Fic family protein	-
PRtIB026_RS21990	4878382..4880055	+	1674	Protein_4309	SulP family inorganic anion transporter	-
PRtIB026_RS21995	4880213..4880533	+	321	WP_203647823.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PRtIB026_RS22000	4880530..4880817	+	288	WP_203647824.1	helix-turn-helix domain-containing protein	Antitoxin
PRtIB026_RS22005	4880856..4882451	-	1596	WP_203647825.1	DUF3327 domain-containing protein	-
PRtIB026_RS22010	4882459..4884822	-	2364	WP_203647826.1	TonB-dependent receptor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-87)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12007.03 Da
• Isoelectric Point: 9.5372

>T37649 WP_203647823.1 NZ_AP023348:4880213-4880533 [Pseudomonas sp. RtIB026]
MLFTETAIFTKRVRELLDDEAYRMLQVRLMMCPDAGEIVEGTGGLRKIRVPANGHGKRGGARIIYYHFVAASQIAMLCIY
PKNEQSDLSATQRKVLKAVIEHWSQP

Nucleotide

• Length: 321 bp

>T37649 NZ_AP023348:4880213-4880533 [Pseudomonas sp. RtIB026]
ATGCTCTTCACCGAAACCGCCATCTTTACCAAACGCGTCAGAGAGTTGCTCGACGATGAGGCCTACCGAATGCTTCAAGT
ACGCCTCATGATGTGCCCCGACGCGGGAGAGATTGTTGAGGGAACCGGCGGCCTGCGCAAGATCCGCGTCCCGGCAAATG
GGCATGGAAAGCGTGGAGGCGCCAGGATCATCTACTACCACTTCGTCGCCGCATCCCAGATTGCCATGCTTTGTATCTAT
CCCAAGAATGAACAGTCAGACCTTAGCGCCACGCAGCGCAAGGTACTGAAAGCTGTTATCGAACACTGGAGCCAGCCATG
A

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10888.38 Da
• Isoelectric Point: 6.4703

>AT37649 WP_203647824.1 NZ_AP023348:4880530-4880817 [Pseudomonas sp. RtIB026]
MSRFFDELMESVEQMDEIIQGQRPPSRQFEIDALQVRSIRKATGLSQARFAEMIDVQVATLRNWEQGRREPTGPAKALLR
AINNDPDHVLKALSH

Nucleotide

• Length: 288 bp

>AT37649 NZ_AP023348:4880530-4880817 [Pseudomonas sp. RtIB026]
ATGAGCCGTTTCTTCGACGAACTGATGGAAAGCGTTGAACAAATGGATGAGATCATCCAAGGTCAACGCCCTCCCTCACG
CCAGTTCGAAATCGACGCCCTCCAGGTCAGGAGCATACGCAAGGCGACCGGGCTATCTCAGGCACGCTTTGCCGAGATGA
TCGATGTGCAGGTGGCCACCTTGCGTAACTGGGAACAGGGACGTCGAGAGCCGACAGGGCCGGCCAAAGCCCTGTTGAGG
GCCATCAACAACGATCCAGATCATGTTCTGAAAGCACTTTCCCACTGA