Detailed information of TA system TA037548

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4719926..4720528
• Replicon: chromosome
• Accession: NZ_AP023315
• Organism: Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain L-4614

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: K5684_RS22925
• Protein ID: WP_001159630.1
• Coordinates: 4720217..4720528 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5684_RS22920
• Protein ID: WP_000362050.1
• Coordinates: 4719926..4720216 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5684_RS22905 (4717419)	4717419..4718321	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
K5684_RS22910 (4718318)	4718318..4718953	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
K5684_RS22915 (4718950)	4718950..4719879	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
K5684_RS22920 (4719926)	4719926..4720216	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
K5684_RS22925 (4720217)	4720217..4720528	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
K5684_RS22930 (4720746)	4720746..4721675	+	930	WP_001127703.1	alpha/beta hydrolase	-
K5684_RS22935 (4721761)	4721761..4722072	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
K5684_RS22940 (4722069)	4722069..4722515	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
K5684_RS22945 (4722530)	4722530..4723471	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
K5684_RS22950 (4723516)	4723516..4723953	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
K5684_RS22955 (4723950)	4723950..4724822	-	873	WP_000921427.1	virulence factor BrkB family protein	-
K5684_RS22960 (4724816)	4724816..4725415	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T37548 WP_001159630.1 NZ_AP023315:c4720528-4720217 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T37548 NZ_AP023315:c4720528-4720217 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT37548 WP_000362050.1 NZ_AP023315:c4720216-4719926 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT37548 NZ_AP023315:c4720216-4719926 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64