Detailed information of TA system TA037263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4772782..4773384
• Replicon: chromosome
• Accession: NZ_AP023291
• Organism: Salmonella enterica subsp. enterica serovar Typhimurium strain L-4126

Toxin (Protein)

• Gene name: higB
• Uniprot ID: C0Q3J8
• Locus tag: K5613_RS23260
• Protein ID: WP_001159630.1
• Coordinates: 4773073..4773384 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: K5613_RS23255
• Protein ID: WP_000362050.1
• Coordinates: 4772782..4773072 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5613_RS23240 (4770275)	4770275..4771177	+	903	WP_000331361.1	formate dehydrogenase subunit beta	-
K5613_RS23245 (4771174)	4771174..4771809	+	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
K5613_RS23250 (4771806)	4771806..4772735	+	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
K5613_RS23255 (4772782)	4772782..4773072	-	291	WP_000362050.1	DNA-binding transcriptional regulator	Antitoxin
K5613_RS23260 (4773073)	4773073..4773384	-	312	WP_001159630.1	cytotoxic translational repressor of toxin-antitoxin stability system	Toxin
K5613_RS23265 (4773602)	4773602..4774531	+	930	WP_001127703.1	alpha/beta hydrolase	-
K5613_RS23270 (4774617)	4774617..4774928	+	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	-
K5613_RS23275 (4774925)	4774925..4775371	+	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	-
K5613_RS23280 (4775386)	4775386..4776327	-	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
K5613_RS23285 (4776372)	4776372..4776809	-	438	WP_000560974.1	D-aminoacyl-tRNA deacylase	-
K5613_RS23290 (4776806)	4776806..4777678	-	873	WP_000921427.1	virulence factor BrkB family protein	-
K5613_RS23295 (4777672)	4777672..4778271	-	600	WP_000965695.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12326.27 Da
• Isoelectric Point: 9.4460

>T37263 WP_001159630.1 NZ_AP023291:c4773384-4773073 [Salmonella enterica subsp. enterica serovar Typhimurium]
MQFIETELFTEDVKKLLDDDEYHKLQVFMAQHPDCGDVIQETGGLRKMRWGARGKGKRSGVRIIYFHRSQRYEIRLLLIY
QKGIKDDLTPQEKAVLRMLNERW

Nucleotide

• Length: 312 bp

>T37263 NZ_AP023291:c4773384-4773073 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10971.59 Da
• Isoelectric Point: 10.6525

>AT37263 WP_000362050.1 NZ_AP023291:c4773072-4772782 [Salmonella enterica subsp. enterica serovar Typhimurium]
MDKVLFERLTQSMSQMNEIIEGTREPSRTFHIDAMKIKEIRQASGLSQSKFAELISVNVDTLRNWEQGRRSPTGPAKALL
RAIANDPRNVIQALRY

Nucleotide

• Length: 291 bp

>AT37263 NZ_AP023291:c4773072-4772782 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• AlphaFold DB: A0A0R9NS64