Detailed information of TA system TA037160

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3667289..3667983
• Replicon: chromosome
• Accession: NZ_AP023286
• Organism: Escherichia coli strain 2018-18.25PC

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: JMH23_RS17625
• Protein ID: WP_001263493.1
• Coordinates: 3667289..3667687 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: JMH23_RS17630
• Protein ID: WP_000554757.1
• Coordinates: 3667690..3667983 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JMH23_RS17595	3662289..3663533	-	1245	WP_000189541.1	esterase FrsA	-
-	3662949..3663029	-	81	NuclAT_10	-	-
-	3662949..3663029	-	81	NuclAT_10	-	-
-	3662949..3663029	-	81	NuclAT_10	-	-
-	3662949..3663029	-	81	NuclAT_10	-	-
JMH23_RS17600	3663625..3664083	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
JMH23_RS17605	3664344..3665801	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
JMH23_RS17610	3665858..3666379	-	522	Protein_3444	peptide chain release factor H	-
JMH23_RS17615	3666378..3666581	-	204	Protein_3445	RNA ligase RtcB family protein	-
JMH23_RS17620	3666827..3667279	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
JMH23_RS17625	3667289..3667687	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
JMH23_RS17630	3667690..3667983	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
JMH23_RS17635	3668035..3669090	-	1056	WP_001226164.1	DNA polymerase IV	-
JMH23_RS17640	3669161..3669946	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
JMH23_RS17645	3669918..3671630	+	1713	Protein_3451	flagellar biosynthesis protein FlhA	-
JMH23_RS17650	3671854..3672351	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3666327..3682569	16242

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T37160 WP_001263493.1 NZ_AP023286:c3667687-3667289 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T37160 NZ_AP023286:c3667687-3667289 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT37160 WP_000554757.1 NZ_AP023286:c3667983-3667690 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT37160 NZ_AP023286:c3667983-3667690 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6