Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1762441..1762943 Replicon chromosome
Accession NZ_AP023286
Organism Escherichia coli strain 2018-18.25PC

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag JMH23_RS08315 Protein ID WP_000767829.1
Coordinates 1762689..1762943 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag JMH23_RS08310 Protein ID WP_001259255.1
Coordinates 1762441..1762692 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JMH23_RS08285 1757620..1758687 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
JMH23_RS08290 1758687..1759757 - 1071 WP_001410397.1 histidinol-phosphate transaminase -
JMH23_RS08295 1759754..1761058 - 1305 WP_001317939.1 histidinol dehydrogenase -
JMH23_RS08300 1761064..1761963 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
JMH23_RS08305 1762109..1762159 - 51 WP_001364200.1 his operon leader peptide -
JMH23_RS08310 1762441..1762692 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
JMH23_RS08315 1762689..1762943 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
JMH23_RS08320 1763026..1763850 + 825 WP_000754763.1 SDR family oxidoreductase -
JMH23_RS08325 1763896..1764825 + 930 WP_000803351.1 LysR family transcriptional regulator -
JMH23_RS08330 1765040..1765102 + 63 WP_010723108.1 membrane protein YoeI -
JMH23_RS08335 1765092..1766450 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
JMH23_RS08340 1766629..1767687 + 1059 WP_023568741.1 transport protein YeeE -
JMH23_RS08345 1767701..1767928 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T37148 WP_000767829.1 NZ_AP023286:1762689-1762943 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T37148 NZ_AP023286:1762689-1762943 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT37148 WP_001259255.1 NZ_AP023286:1762441-1762692 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT37148 NZ_AP023286:1762441-1762692 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References