Detailed information of TA system TA037077

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3804715..3805331
• Replicon: chromosome
• Accession: NZ_AP023242
• Organism: Mesorhizobium sp. 113-1-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: JN279_RS18420
• Protein ID: WP_202353563.1
• Coordinates: 3805035..3805331 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: JN279_RS18415
• Protein ID: WP_096447474.1
• Coordinates: 3804715..3805023 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN279_RS18400	3800643..3801821	-	1179	WP_096447480.1	lytic murein transglycosylase	-
JN279_RS18405	3802231..3803031	-	801	WP_096447478.1	glycoside hydrolase family 25 protein	-
JN279_RS18410	3803320..3804699	+	1380	WP_096447476.1	serine hydrolase	-
JN279_RS18415	3804715..3805023	-	309	WP_096447474.1	HigA family addiction module antidote protein	Antitoxin
JN279_RS18420	3805035..3805331	-	297	WP_202353563.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
JN279_RS18425	3805451..3806134	-	684	WP_202353564.1	class I SAM-dependent methyltransferase	-
JN279_RS18430	3806131..3807429	-	1299	WP_202353565.1	DUF3419 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11740.19 Da
• Isoelectric Point: 6.0777

>T37077 WP_202353563.1 NZ_AP023242:c3805331-3805035 [Mesorhizobium sp. 113-1-2]
MIVDFRDEWLRAFFVQDTHSRNIPSDLESRLFRKLQMIDDAMIDQDLRVPPSNHFEKLSGNLEGFHSIRVNKQWRLIFRW
DGGRGEASDIYLDDHSYK

Nucleotide

• Length: 297 bp

>T37077 NZ_AP023242:c3805331-3805035 [Mesorhizobium sp. 113-1-2]
ATGATCGTCGACTTTAGGGACGAATGGCTGCGGGCCTTCTTTGTGCAGGATACCCATTCTCGTAACATTCCGTCCGATCT
CGAAAGCCGCTTGTTCCGCAAACTCCAGATGATTGACGACGCTATGATCGATCAGGATTTGCGTGTGCCGCCCAGCAACC
ATTTCGAGAAGTTGAGCGGCAATCTCGAAGGCTTCCATTCGATCCGCGTCAACAAGCAGTGGCGACTGATCTTCCGCTGG
GACGGCGGTCGCGGTGAAGCGTCGGACATCTATCTCGACGACCACAGCTATAAATAA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11385.15 Da
• Isoelectric Point: 9.4051

>AT37077 WP_096447474.1 NZ_AP023242:c3805023-3804715 [Mesorhizobium sp. 113-1-2]
MLMTARKPATVGEILTEEFMQPLGLTQAALAEAMGVQRKHVNELCNDRRNVTAATALILARVFGNSPDFWLNVQRRNDLW
AVMNSPDERARVDRAKPLPTAP

Nucleotide

• Length: 309 bp

>AT37077 NZ_AP023242:c3805023-3804715 [Mesorhizobium sp. 113-1-2]
ATGTTGATGACCGCACGCAAGCCAGCAACGGTTGGCGAAATTCTCACCGAGGAATTCATGCAACCGCTCGGCTTGACGCA
GGCGGCTTTGGCCGAGGCGATGGGCGTTCAGCGCAAGCATGTCAACGAATTGTGCAACGACCGCCGCAACGTGACGGCCG
CTACCGCACTCATTCTGGCCCGGGTGTTTGGCAACAGCCCGGACTTCTGGCTGAATGTCCAACGCCGCAACGATCTTTGG
GCGGTTATGAATTCGCCCGACGAACGGGCGCGTGTTGACCGCGCCAAGCCTTTGCCAACAGCCCCATAG