Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1837305..1837807 Replicon chromosome
Accession NZ_AP023237
Organism Escherichia coli strain F070

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag FJI53_RS08725 Protein ID WP_000767829.1
Coordinates 1837553..1837807 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag FJI53_RS08720 Protein ID WP_001259255.1
Coordinates 1837305..1837556 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FJI53_RS08695 1832482..1833549 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
FJI53_RS08700 1833549..1834619 - 1071 WP_053908449.1 histidinol-phosphate transaminase -
FJI53_RS08705 1834616..1835920 - 1305 WP_053908451.1 histidinol dehydrogenase -
FJI53_RS08710 1835926..1836825 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
FJI53_RS08715 1836971..1837021 - 51 WP_001364200.1 his operon leader peptide -
FJI53_RS08720 1837305..1837556 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
FJI53_RS08725 1837553..1837807 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
FJI53_RS08730 1837890..1838714 + 825 WP_053908453.1 SDR family oxidoreductase -
FJI53_RS08735 1838760..1839689 + 930 WP_053908455.1 LysR family transcriptional regulator -
FJI53_RS08740 1839904..1839966 + 63 WP_010723108.1 membrane protein YoeI -
FJI53_RS08745 1839956..1841314 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
FJI53_RS08750 1841493..1842551 + 1059 WP_053908457.1 transport protein YeeE -
FJI53_RS08755 1842565..1842792 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T37051 WP_000767829.1 NZ_AP023237:1837553-1837807 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T37051 NZ_AP023237:1837553-1837807 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT37051 WP_001259255.1 NZ_AP023237:1837305-1837556 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT37051 NZ_AP023237:1837305-1837556 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References