Detailed information of TA system TA037040

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 53974..54243
• Replicon: plasmid pYJ6-NDM5
• Accession: NZ_AP023236
• Organism: Escherichia coli strain YJ6

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HXS64_RS22695
• Protein ID: WP_001312861.1
• Coordinates: 54085..54243 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	53974..54039 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS64_RS22665	49684..50211	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HXS64_RS22670	50269..50502	+	234	WP_000006003.1	DUF905 family protein	-
HXS64_RS22675	50563..52586	+	2024	Protein_63	ParB/RepB/Spo0J family partition protein	-
HXS64_RS22680	52655..53089	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HXS64_RS22685	53086..53805	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	53817..54041	+	225	NuclAT_0	-	-
-	53817..54041	+	225	NuclAT_0	-	-
-	53817..54041	+	225	NuclAT_0	-	-
-	53817..54041	+	225	NuclAT_0	-	-
HXS64_RS22690	53826..54005	-	180	WP_001309233.1	hypothetical protein	-
-	53974..54039	+	66	-	-	Antitoxin
HXS64_RS22695	54085..54243	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HXS64_RS22700	54481..54858	-	378	Protein_68	hypothetical protein	-
HXS64_RS22705	55158..55454	+	297	WP_001272251.1	hypothetical protein	-
HXS64_RS22710	55565..56386	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HXS64_RS22715	56683..57285	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HXS64_RS22720	57608..57991	+	384	WP_001354030.1	relaxosome protein TraM	-
HXS64_RS22725	58185..58856	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HXS64_RS22730	58993..59220	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / blaTEM-1B / rmtB / blaNDM-5 / sul1 / qacE / aadA2 / dfrA12	-	1..94613	94613

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T37040 WP_001312861.1 NZ_AP023236:54085-54243 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T37040 NZ_AP023236:54085-54243 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 66 bp

>AT37040 NZ_AP023236:53974-54039 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin