Detailed information of TA system TA037035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3931702..3932396
• Replicon: chromosome
• Accession: NZ_AP023235
• Organism: Escherichia coli strain YJ6

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: HXS64_RS19075
• Protein ID: WP_001263493.1
• Coordinates: 3931702..3932100 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: HXS64_RS19080
• Protein ID: WP_000554757.1
• Coordinates: 3932103..3932396 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS64_RS19045	3926702..3927946	-	1245	WP_000189541.1	esterase FrsA	-
-	3927362..3927442	-	81	NuclAT_12	-	-
-	3927362..3927442	-	81	NuclAT_12	-	-
-	3927362..3927442	-	81	NuclAT_12	-	-
-	3927362..3927442	-	81	NuclAT_12	-	-
HXS64_RS19050	3928038..3928496	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HXS64_RS19055	3928757..3930214	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
HXS64_RS19060	3930271..3930792	-	522	Protein_3711	peptide chain release factor H	-
HXS64_RS19065	3930791..3930994	-	204	Protein_3712	RNA ligase RtcB family protein	-
HXS64_RS19070	3931240..3931692	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
HXS64_RS19075	3931702..3932100	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HXS64_RS19080	3932103..3932396	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HXS64_RS19085	3932448..3933503	-	1056	WP_001226164.1	DNA polymerase IV	-
HXS64_RS19090	3933574..3934359	-	786	WP_072132640.1	putative lateral flagellar export/assembly protein LafU	-
HXS64_RS19095	3934331..3936043	+	1713	Protein_3718	flagellar biosynthesis protein FlhA	-
HXS64_RS19100	3936259..3936756	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T37035 WP_001263493.1 NZ_AP023235:c3932100-3931702 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T37035 NZ_AP023235:c3932100-3931702 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT37035 WP_000554757.1 NZ_AP023235:c3932396-3932103 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT37035 NZ_AP023235:c3932396-3932103 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6