Detailed information of TA system TA037012

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46678..46932
• Replicon: plasmid pYJ4-a
• Accession: NZ_AP023232
• Organism: Escherichia coli strain YJ4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HXS62_RS23780
• Protein ID: WP_032215358.1
• Coordinates: 46726..46932 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	46678..46739 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS62_RS23750	42438..43184	+	747	WP_052273601.1	conjugal transfer pilus acetylase TraX	-
HXS62_RS23755	43239..43799	+	561	WP_001567328.1	fertility inhibition protein FinO	-
HXS62_RS23760	43930..44139	+	210	WP_039023209.1	hypothetical protein	-
HXS62_RS23765	44551..45177	+	627	WP_029702149.1	hypothetical protein	-
HXS62_RS23770	45329..45898	+	570	WP_029702150.1	DUF2726 domain-containing protein	-
HXS62_RS23775	46273..46539	+	267	WP_087757657.1	hypothetical protein	-
-	46678..46739	-	62	NuclAT_0	-	Antitoxin
-	46678..46739	-	62	NuclAT_0	-	Antitoxin
-	46678..46739	-	62	NuclAT_0	-	Antitoxin
-	46678..46739	-	62	NuclAT_0	-	Antitoxin
HXS62_RS23780	46726..46932	+	207	WP_032215358.1	type I toxin-antitoxin system Hok family toxin	Toxin
HXS62_RS23785	47216..47473	+	258	WP_000083833.1	replication regulatory protein RepA	-
HXS62_RS23790	47709..47783	+	75	WP_001365705.1	RepA leader peptide Tap	-
HXS62_RS23795	47776..48604	+	829	Protein_56	incFII family plasmid replication initiator RepA	-
HXS62_RS23800	49306..49446	+	141	WP_001333237.1	hypothetical protein	-
HXS62_RS23805	49872..50204	+	333	WP_072134990.1	CPBP family intramembrane metalloprotease	-
HXS62_RS23810	50297..50554	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HXS62_RS23815	50487..50888	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HXS62_RS23820	51137..51553	+	417	Protein_61	IS1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	qnrS1 / blaCTX-M-15 / blaTEM-1B / tet(B)	-	1..130952	130952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7778.27 Da
• Isoelectric Point: 8.8807

>T37012 WP_032215358.1 NZ_AP023232:46726-46932 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGVLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T37012 NZ_AP023232:46726-46932 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT37012 NZ_AP023232:c46739-46678 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin