Detailed information of TA system TA036990

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1116723..1117417
• Replicon: chromosome
• Accession: NZ_AP023230
• Organism: Escherichia coli strain YJ4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: HXS62_RS05395
• Protein ID: WP_001263489.1
• Coordinates: 1116723..1117121 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HXS62_RS05400
• Protein ID: WP_000554758.1
• Coordinates: 1117124..1117417 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1112311..1112391	-	81	NuclAT_12	-	-
-	1112311..1112391	-	81	NuclAT_12	-	-
-	1112311..1112391	-	81	NuclAT_12	-	-
-	1112311..1112391	-	81	NuclAT_12	-	-
HXS62_RS05370	1112987..1113445	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HXS62_RS05375	1113706..1115163	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
HXS62_RS05380	1115220..1115741	-	522	Protein_1043	peptide chain release factor H	-
HXS62_RS05385	1115737..1115943	-	207	Protein_1044	RtcB family protein	-
HXS62_RS05390	1116261..1116713	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
HXS62_RS05395	1116723..1117121	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HXS62_RS05400	1117124..1117417	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HXS62_RS05405	1117469..1118524	-	1056	WP_001226164.1	DNA polymerase IV	-
HXS62_RS05410	1118595..1119380	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HXS62_RS05415	1119352..1121064	+	1713	Protein_1050	flagellar biosynthesis protein FlhA	-
HXS62_RS05420	1121288..1121785	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA	1028327..1133664	105337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T36990 WP_001263489.1 NZ_AP023230:c1117121-1116723 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T36990 NZ_AP023230:c1117121-1116723 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT36990 WP_000554758.1 NZ_AP023230:c1117417-1117124 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT36990 NZ_AP023230:c1117417-1117124 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3