Detailed information of TA system TA036976

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33595..33849
• Replicon: plasmid pYJ3-a
• Accession: NZ_AP023228
• Organism: Escherichia coli strain YJ3

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: HXS63_RS23595
• Protein ID: WP_015387399.1
• Coordinates: 33595..33801 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	33788..33849 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS63_RS23565	28842..29753	-	912	WP_000440183.1	carbamate kinase	-
HXS63_RS23570	29764..30984	-	1221	WP_000410951.1	arginine deiminase	-
HXS63_RS23575	31877..32359	+	483	Protein_42	VENN motif pre-toxin domain-containing protein	-
HXS63_RS23580	32305..32751	-	447	Protein_43	incFII family plasmid replication initiator RepA	-
HXS63_RS23585	32744..32818	-	75	WP_032336874.1	RepA leader peptide Tap	-
HXS63_RS23590	33054..33311	-	258	WP_000083833.1	replication regulatory protein RepA	-
HXS63_RS23595	33595..33801	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	33788..33849	+	62	NuclAT_2	-	Antitoxin
-	33788..33849	+	62	NuclAT_2	-	Antitoxin
-	33788..33849	+	62	NuclAT_2	-	Antitoxin
-	33788..33849	+	62	NuclAT_2	-	Antitoxin
HXS63_RS23600	33988..34170	-	183	WP_000968309.1	hypothetical protein	-
HXS63_RS23605	34271..34876	+	606	Protein_48	IS1 family transposase	-
HXS63_RS23610	34914..36486	-	1573	Protein_49	IS66 family transposase	-
HXS63_RS23615	36506..36853	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
HXS63_RS23620	36853..37534	-	682	Protein_51	transposase	-
HXS63_RS23625	37589..37678	+	90	Protein_52	IS1 family transposase	-
HXS63_RS23630	37979..38191	-	213	WP_005012601.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA5 / qacE / sul1 / mph(A) / erm(B) / sitABCD / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / tet(B) / catA1	iutA / iucD / iucC / iucB / iucA	1..170909	170909
	-	inside	Integron	-	iutA / iucD / iucC / iucB / iucA	1..170909	170908

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T36976 WP_015387399.1 NZ_AP023228:c33801-33595 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T36976 NZ_AP023228:c33801-33595 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT36976 NZ_AP023228:33788-33849 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin