Detailed information of TA system TA036972

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3775385..3776079
• Replicon: chromosome
• Accession: NZ_AP023226
• Organism: Escherichia coli strain YJ3

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: HXS63_RS18120
• Protein ID: WP_001263489.1
• Coordinates: 3775385..3775783 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HXS63_RS18125
• Protein ID: WP_000554758.1
• Coordinates: 3775786..3776079 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3770973..3771053	-	81	NuclAT_12	-	-
-	3770973..3771053	-	81	NuclAT_12	-	-
-	3770973..3771053	-	81	NuclAT_12	-	-
-	3770973..3771053	-	81	NuclAT_12	-	-
HXS63_RS18095	3771649..3772107	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
HXS63_RS18100	3772368..3773825	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
HXS63_RS18105	3773882..3774403	-	522	Protein_3531	peptide chain release factor H	-
HXS63_RS18110	3774399..3774605	-	207	Protein_3532	RtcB family protein	-
HXS63_RS18115	3774923..3775375	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
HXS63_RS18120	3775385..3775783	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HXS63_RS18125	3775786..3776079	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HXS63_RS18130	3776131..3777186	-	1056	WP_001226164.1	DNA polymerase IV	-
HXS63_RS18135	3777257..3778042	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
HXS63_RS18140	3778014..3779726	+	1713	Protein_3538	flagellar biosynthesis protein FlhA	-
HXS63_RS18145	3779950..3780447	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA	3760355..3790664	30309

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T36972 WP_001263489.1 NZ_AP023226:c3775783-3775385 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T36972 NZ_AP023226:c3775783-3775385 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT36972 WP_000554758.1 NZ_AP023226:c3776079-3775786 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT36972 NZ_AP023226:c3776079-3775786 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3