Detailed information of TA system TA036946

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 23541..23780
• Replicon: plasmid pYJ1-NDM5
• Accession: NZ_AP023225
• Organism: Escherichia coli strain YJ1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HXS59_RS23365
• Protein ID: WP_023144756.1
• Coordinates: 23541..23675 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	23720..23780 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HXS59_RS23340	19239..20096	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
HXS59_RS23345	20089..20163	-	75	WP_031943482.1	RepA leader peptide Tap	-
HXS59_RS23910	20160..20294	-	135	Protein_21	protein CopA/IncA	-
HXS59_RS23350	20525..22069	+	1545	WP_176486343.1	IS21-like element ISEc12 family transposase	-
HXS59_RS23355	22084..22830	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
HXS59_RS23360	22990..23244	-	255	WP_000083850.1	replication regulatory protein RepA	-
HXS59_RS23365	23541..23675	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	23720..23780	+	61	NuclAT_0	-	Antitoxin
-	23720..23780	+	61	NuclAT_0	-	Antitoxin
-	23720..23780	+	61	NuclAT_0	-	Antitoxin
-	23720..23780	+	61	NuclAT_0	-	Antitoxin
HXS59_RS23370	23747..24033	-	287	Protein_26	DUF2726 domain-containing protein	-
HXS59_RS23375	24546..24758	-	213	WP_013023861.1	hypothetical protein	-
HXS59_RS23380	24889..25449	-	561	WP_000139341.1	fertility inhibition protein FinO	-
HXS59_RS23385	25504..26250	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
HXS59_RS23390	26270..28663	-	2394	Protein_30	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(B) / sul1 / qacE / aadA2 / dfrA12 / blaNDM-5 / aadA5 / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2	-	1..108281	108281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T36946 WP_023144756.1 NZ_AP023225:c23675-23541 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T36946 NZ_AP023225:c23675-23541 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT36946 NZ_AP023225:23720-23780 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin